Steady-state electrochemical determination of lipidic nanotube diameter utilizing an artificial cell model.
Steady-state electrochemical determination of lipidic nanotube diameter utilizing an artificial cell model.
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DOI：
10.1021/ac902282d
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发表时间：
2010-02-01
影响因子：
7.4
通讯作者：
Ewing, Andrew G.
中科院分区：
文献类型：
--
作者：
Adams, Kelly L.;Engelbrektsson, Johan;Voinova, Marina;Zhang, Bo;Eves, Daniel J.;Karlsson, Roger;Heien, Michael L.;Cans, Ann-Sofie;Ewing, Andrew G.
通过利用稳态电化学测量的能力，我们已经测量了使用Fick第一扩散定律结合施加的线性浓度梯度的电活性分子在纳米管的长度的脂质纳米管的内径。Fick定律以这种方式用于提供纳米管直径与可测量的实验参数Δi（电流变化）和纳米管长度之间的直接关系。邻苯二酚用于确定归因于其流出纳米管的Δi。比较作为纳米管长度的函数的纳米管直径显示，膜弹性能在确定纳米管的尺寸中起重要作用，并且当管连接到两个囊泡的任一端或一端连接到囊泡而另一端连接到移液管尖端时，膜弹性能是不同的。我们假设颈部区域之间的排斥相互作用可以用来解释观察到的趋势。这种基于弹性能量考虑的理论方法提供了与实验数据一致的定性描述。
By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick's first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick's law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters Δi (change in current) and nanotube length. Catechol was used to determine the Δi attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipette tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.
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影响因子：
3.3
作者：
Karlsson, A;Sott, K;Orwar, O
通讯作者：
Orwar, O
DOI：
10.1073/pnas.232702599
发表时间：
2003-01-21
影响因子：
11.1
作者：
Cans, AS;Wittenberg, N;Ewing, A
通讯作者：
Ewing, A
影响因子：
4.1
作者：
Durgbanshi, A;Kok, WT
通讯作者：
Kok, WT
影响因子：
2.9
作者：
Lauf, U;Fahr, A;Ulrich, AS
通讯作者：
Ulrich, AS
影响因子：
8.6
作者：
MICHALET, X;BENSIMON, D;FOURCADE, B
通讯作者：
FOURCADE, B