A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy.

A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy.
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DOI:
10.4161/onci.27258
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发表时间:
2014-01-01
期刊:
影响因子:
7.2
通讯作者:
Muraguchi A
Muraguchi A
中科院分区:
医学2区
文献类型:
--
作者:
Kobayashi E;Kishi H;Muraguchi A

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以T细胞受体(TCR)为基础的基因免疫治疗已成为治疗多种恶性肿瘤的一种有前途的方法。我们最近报道了一种高效的TCR编码基因克隆和功能评估系统。该系统被我们命名为hTEC10,允许在不到10天的时间内确定TCR抗原的特异性,因此可能构成一个快速而强大的平台,用于开发基于TCR的新的抗癌疗法。
T-cell receptor (TCR)-based gene immunotherapy has emerged as a promising approach for the treatment of multiple malignancies. We have recently reported an efficient system for the cloning and functional evaluation of TCR-coding cDNAs. This system, which we named hTEC10, allows for the determination of TCR antigen specificity in less than 10 days, and may therefore constitute a fast and powerful platform for the development of new TCR-based anticancer therapies.
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