Development of an in vitro 3D tumor model to study therapeutic efficiency of an anticancer drug.
Development of an in vitro 3D tumor model to study therapeutic efficiency of an anticancer drug.
复制标题
开发体外3D肿瘤模型，以研究抗癌药物的治疗效率。
DOI：
10.1021/mp300595a
复制
发表时间：
2013-06-03
影响因子：
4.9
通讯作者：
Park K
中科院分区：
文献类型：
--
作者：
Shin CS;Kwak B;Han B;Park K
三维（3D）细胞培养的重要性和优势已得到广泛认可。在3D培养系统中培养的肿瘤细胞作为多细胞肿瘤球体（MTS）可以弥合体外和体内抗癌药物评价之间的差距。体外三维肿瘤模型能够提供体内药物疗效的密切预测，将提高我们的理解，设计和开发更好的药物输送系统。在这里，我们开发了一种体外3D肿瘤模型，通过调整水凝胶模板策略，在含有微孔的水凝胶支架中培养均匀大小的球状体。体外3D肿瘤模型是为了紧密模拟体内实体肿瘤及其微环境，用于评价抗癌药物递送系统。在水凝胶支架中培养的MTS用于检查培养条件对药物反应的影响。将从支架释放的游离MTS转移到微流体通道以模拟动态体内微环境。体外3D肿瘤模型模拟体内微环境的生物学相关参数，如细胞-细胞和细胞-ECM相互作用，以及动态环境，将是检查抗癌药物效率和靶向特异性的有价值的装置。这些模型具有提供体内相关信息以改进和优化用于有效化疗的药物递送系统的潜力。
The importance and advantages of three-dimensional (3D) cell cultures have been well-recognized. Tumor cells cultured in a 3D culture system as multicellular tumor spheroids (MTS) can bridge the gap between in vitro and in vivo anticancer drug evaluations. An in vitro 3D tumor model capable of providing close predictions of in vivo drug efficacy will enhance our understanding, design, and development of better drug delivery systems. Here, we developed an in vitro 3D tumor model by adapting the hydrogel template strategy to culture uniformly sized spheroids in a hydrogel scaffold containing microwells. The in vitro 3D tumor model was to closely simulate an in vivo solid tumor and its microenvironment for evaluation of anticancer drug delivery systems. MTS cultured in the hydrogel scaffold are used to examine the effect of culture conditions on the drug responses. Free MTS released from the scaffold are transferred to a microfluidic channel to simulate a dynamic in vivo microenvironment. The in vitro 3D tumor model that mimics biologically relevant parameters of in vivo microenvironments such as cell-cell and cell-ECM interactions, and a dynamic environment would be a valuable device to examine efficiency of anticancer drug and targeting specificity. These models have potential to provide in vivo correlated information to improve and optimize drug delivery systems for an effective chemotherapy.
登录
查看更多内容
影响因子：
6.4
作者：
Cariati, Massimiliano;Naderi, Ali;Purushotham, Anand D.
通讯作者：
Purushotham, Anand D.
影响因子：
48
作者：
Fischbach, Claudia;Chen, Ruth;Mooney, David J.
通讯作者：
Mooney, David J.
影响因子：
3.8
作者：
Ng, Chee Ping;Pun, Suzie Hwang
通讯作者：
Pun, Suzie Hwang
DOI：
10.1007/978-1-61779-052-2_12
发表时间：
2011-01-01
期刊：
BIOMEDICAL NANOETECHNOLOGY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Acharya, Ghanashyam;McDermott, Matthew;Park, Kinam
通讯作者：
Park, Kinam
影响因子：
48
作者：
Lee, Genee Y.;Kenny, Paraic A.;Bissell, Mina J.
通讯作者：
Bissell, Mina J.