Astrocytes and oligodendrocytes in grey and white matter regions of the brain metabolize fatty acids.
Astrocytes and oligodendrocytes in grey and white matter regions of the brain metabolize fatty acids.
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DOI：
10.1038/s41598-017-11103-5
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发表时间：
2017-09-07
影响因子：
4.6
通讯作者：
Kuerschner L
中科院分区：
文献类型：
--
作者：
Hofmann K;Rodriguez-Rodriguez R;Gaebler A;Casals N;Scheller A;Kuerschner L
哺乳动物大脑的灰质和白色区由神经元和神经胶质细胞组成。在神经胶质细胞中，两种大胶质细胞少突胶质细胞和星形胶质细胞是最丰富的细胞类型。虽然少突胶质细胞的主要功能是形成富含脂质的髓鞘结构，但星形胶质细胞的异质组在脑发育和稳态中发挥多种重要作用。脑脂质稳态涉及通过局部脂质代谢合成特定的脑脂质体。在这项研究中，我们研究了小鼠脑灰质和白色区的脂肪酸摄取和脂质生物合成。主要调查结果如下：（i）白色物质少突胶质细胞和星形胶质细胞摄取饱和和不饱和脂肪酸，（ii）不同的灰质区域显示不同的脂质标记强度，（iii）内侧缰（一种上丘脑灰质结构）和其中的少突胶质细胞和星形胶质细胞被脂肪酸靶向，和（iv）在内侧缰中，在面向心室的细胞中发现含有中性脂质的脂滴，但在缰实质中检测不到。我们的数据表明少突胶质细胞和星形胶质细胞在大脑白色和灰质区域的局部脂质代谢中发挥作用。
The grey and white matter regions of the mammalian brain consist of both neurons and neuroglial cells. Among the neuroglia, the two macroglia oligodendrocytes and astrocytes are the most abundant cell types. While the major function of oligodendrocytes is the formation of the lipid-rich myelin structure, the heterogeneous group of astrocytes fulfils a multitude of important roles in cerebral development and homeostasis. Brain lipid homeostasis involves the synthesis of a specific cerebral lipidome by local lipid metabolism. In this study we have investigated the fatty acid uptake and lipid biosynthesis in grey and white matter regions of the murine brain. Key findings were: (i) white matter oligodendrocytes and astrocytes take up saturated and unsaturated fatty acids, (ii) different grey matter regions show varying lipid labelling intensities, (iii) the medial habenula, an epithalamic grey matter structure, and the oligodendrocytes and astrocytes therein are targeted by fatty acids, and (iv) in the medial habenula, the neutral lipid containing lipid droplets are found in cells facing the ventricle but undetectable in the habenular parenchyma. Our data indicate a role for oligodendrocytes and astrocytes in local lipid metabolism of white and grey matter regions in the brain.
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影响因子：
5.3
作者：
Jahn HM;Scheller A;Kirchhoff F
通讯作者：
Kirchhoff F
影响因子：
3.5
作者：
Leone, DP;Genoud, S;Suter, U
通讯作者：
Suter, U
DOI：
10.3791/50326
发表时间：
2013-05-12
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
DeVos SL;Miller TM
通讯作者：
Miller TM
影响因子：
64.8
作者：
Nguyen, Long N.;Ma, Dongliang;Silver, David L.
通讯作者：
Silver, David L.
影响因子：
2.5
作者：
Azevedo, Frederico A. C.;Carvalho, Ludmila R. B.;Herculano-Houzel, Suzana
通讯作者：
Herculano-Houzel, Suzana