Effect of pH and monovalent cations on the ionization state of phosphatidylglycerol in monolayers. An experimental (surface potential) and theoretical (Gouy-Chapman) approach.
Effect of pH and monovalent cations on the ionization state of phosphatidylglycerol in monolayers. An experimental (surface potential) and theoretical (Gouy-Chapman) approach.
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pH 值和单价阳离子对单层磷脂酰甘油电离状态的影响。
DOI：
10.1111/j.1432-1033.1983.tb07599.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. Tocanne
中科院分区：
文献类型：
--
作者：
F. Lakhdar‐Ghazal;Jean;J. Tocanne
本文研究了酸性磷脂磷脂酰甘油的电离，测量了磷脂单分子膜的表面电势随水相pH和单价阳离子(Li、Na、Cs)浓度的变化。结果表明，只要考虑阳离子在膜表面的吸附，实验数据可以用最简单的Gouy-Chapman理论来解释。这使我们能够预测在亚相中给定离子条件下脂类的电离状态。在pH 4以上，亚相离子浓度大于10 mM，或在pH 5.6时，离子浓度大于0.1 mM，磷脂酰甘油完全去质子化。在我们理论方法的范围内，阳离子与脂类的缔合常数在0.1-0.6M-1左右。
Ionization of the acidic phospholipid phosphatidylglycerol has been studied by measuring the surface potential of monomolecular films of the lipid as a function of the aqueous subphase pH and the concentration of monovalent cations (Li, Na, Cs). It is shown that the experimental data can be interpreted by means of the Gouy-Chapman theory in its simplest formulation, provided an adsorption of cations at the membrane surface is accounted for. This allows us to predict the ionization state of the lipid for given ionic conditions in the subphase. Above pH 4, for subphase ion concentration higher than 10 mM, or for ion concentrations above 0.1 mM at pH 5.6, phosphatidylglycerol is fully deprotonated. Within the limits of our theoretical approach, association constants of the cations to the lipid lie around 0.1-0.6 M-1.
DOI：
10.1016/0005-2736(81)90199-1
发表时间：
1981-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
LAU, A;MCLAUGHLIN, A;MCLAUGHLIN, S
通讯作者：
MCLAUGHLIN, S