The biodiversity of Lactobacillus spp. from Iranian raw milk Motal cheese and antibacterial evaluation based on bacteriocin-encoding genes.
The biodiversity of Lactobacillus spp. from Iranian raw milk Motal cheese and antibacterial evaluation based on bacteriocin-encoding genes.
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DOI：
10.1186/s13568-017-0474-2
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发表时间：
2017-09-18
期刊：
影响因子：
3.7
通讯作者：
Edalatian Dovom MR
中科院分区：
文献类型：
--
作者：
Azizi F;Habibi Najafi MB;Edalatian Dovom MR
乳酸杆菌作为乳酸菌中最大的类群，能产生大量的有机酸、脂肪酸、氨、过氧化氢、双乙酰、细菌素等抗菌代谢产物，抑制致病菌的生长，延长食品的货架期。本研究的目的是鉴定乳杆菌属。分离自伊朗生乳Motal奶酪，并检测分离的乳杆菌属菌株中表现出抗微生物活性的细菌素基因的存在。为此目的，从达什特-莫甘地区，伊朗，6 Motal奶酪样品进行了微生物表征。19种乳酸菌分离并随后基于生物化学和分子方法鉴定。根据菌株的测序结果，乳酸杆菌属（Lactobacillus spp.）主要由短乳杆菌、植物乳杆菌、干酪乳杆菌和布氏乳杆菌组成。然后评价鉴定的分离株对大肠杆菌ATCC 25922、无害李斯特菌ATCC 33090和金黄色葡萄球菌ATCC 25923的抗微生物活性。用细菌素基因特异性引物进行PCR分析，结果表明所有植物乳杆菌分离株中均含有植物菌素A和植物菌素EF，5株短乳杆菌分离株中含有短菌素174 A，而在所有检测的分离株中均未观察到Pediocin PA-1基因。因此，可以得出结论，产细菌素的分离物可以被推荐为合适的候选人，用于发酵和非发酵产品的生产的发酵剂，发酵剂或抗菌剂。
Lactobacilli, as the largest group of lactic acid bacteria, produce large amounts of antimicrobial metabolites such as organic acids, fatty acids, ammonia, hydrogen peroxide, diacetyl and bacteriocin, which inhibit the growth of pathogenic bacteria and increase shelf life of food. The aim of this study was to identify the Lactobacillus spp. isolated from Iranian raw milk Motal cheese and to detect the presence of bacteriocin genes in the isolated Lactobacillus strains exhibiting antimicrobial activity. For this purpose, 6 Motal cheese samples from Dasht-e-Moghan region, Iran, were subjected to microbial characterization. Nineteen Lactobacillus spp. were isolated and subsequently identified based on biochemical and molecular methods. According to the sequencing of isolates, Lactobacillus spp. consisted primarily of Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus casei and Lactobacillus buchneri. The identified isolates were then evaluated for antimicrobial activity against Escherichia coli ATCC 25922, Listeria innocua ATCC 33090 and Staphylococcus aureus ATCC 25923. The results of PCR analysis using specific primers of genes encoding Bacteriocin, revealed the presence of Plantaricin A and Plantaricin EF in all Lactobacillus plantarum isolates and Brevicin 174A in 5 of Lactobacillus brevis isolates, whereas the gene encoding Pediocin PA-1 was not observed in any of examined isolates. It is therefore concluded that bacteriocinogenic isolates could be recommended as suitable candidates to be used as starter, adjunct-starter or antimicrobial agents for production of fermented and non-fermented products.
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DOI：
10.1016/j.ijfoodmicro.2014.07.001
发表时间：
2014-10-01
影响因子：
5.4
作者：
Bunesova, Vera;Killer, Jiri;Kmet, Vladimir
通讯作者：
Kmet, Vladimir
DOI：
10.1016/j.ijfoodmicro.2009.09.023
发表时间：
2009-11-30
影响因子：
5.4
作者：
Alegria, Angel;Alvarez-Martin, Pablo;Mayo, Baltasar
通讯作者：
Mayo, Baltasar
DOI：
10.1016/s2222-1808(15)60819-2
发表时间：
2015-01-01
期刊：
Asian Pacific Journal of Tropical Disease
影响因子：
--
作者：
Chaalel, A.;Riazi, A.;Thonart, P.
通讯作者：
Thonart, P.
影响因子：
5.3
作者：
Engelhardt, Tekla;Albano, Helena;Teixeira, Paula
通讯作者：
Teixeira, Paula
影响因子：
0.8
作者：
Jokovic, Natasa;Vukasinovic, Maja;Topisirovic, L.
通讯作者：
Topisirovic, L.