Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes.
Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes.
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DOI：
10.3389/fpls.2021.649001
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发表时间：
2021
影响因子：
5.6
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Guo R;Zhao L;Zhang K;Lu H;Bhanbhro N;Yang C
盐生植物和糖生植物对高盐的耐受性存在明显差异。然而，这种分化背后的遗传机制仍不清楚。为了揭示这些机制，我们调查了耐盐基因家族的进化，通过比较基因组分析之间的模式盐生植物星星草和糖生禾本科植物，并比较其转录和生理响应的盐胁迫。盐胁迫下，细花偃麦草根中K+浓度略有增加，而糖生禾本科植物根中K+浓度显著降低，这为细花偃麦草与糖生禾本科植物耐盐性差异提供了生理学解释。值得注意的是，在进化过程中，一些来自星星草的K+吸收基因家族经历了家族扩张和正选择。该基因家族的扩展和K+吸收基因的表达增加促进了盐胁迫下星星草根系K+的积累，降低了Na+的毒害。正选择的星星草K+吸收基因可能进化出了新的功能，有助于星星草耐盐性的发展。此外，参与磷酸戊糖途径、蔗糖合成和类黄酮合成的基因家族的扩展有助于星星草适应高盐环境。
Halophytes and glycophytes exhibit clear differences in their tolerance to high levels of salinity. The genetic mechanisms underlying this differentiation, however, remain unclear. To unveil these mechanisms, we surveyed the evolution of salinity-tolerant gene families through comparative genomic analyses between the model halophyte Puccinellia tenuiflora and glycophytic Gramineae plants, and compared their transcriptional and physiological responses to salinity stress. Under salinity stress, the K+ concentration in the root was slightly enhanced in P. tenuiflora, but it was greatly reduced in the glycophytic Gramineae plants, which provided a physiological explanation for differences in salinity tolerance between P. tenuiflora and these glycophytes. Interestingly, several K+ uptake gene families from P. tenuiflora experienced family expansion and positive selection during evolutionary history. This gene family expansion and the elevated expression of K+ uptake genes accelerated K+ accumulation and decreased Na+ toxicity in P. tenuiflora roots under salinity stress. Positively selected P. tenuiflora K+ uptake genes may have evolved new functions that contributed to development of P. tenuiflora salinity tolerance. In addition, the expansion of the gene families involved in pentose phosphate pathway, sucrose biosynthesis, and flavonoid biosynthesis assisted the adaptation of P. tenuiflora to survival under high salinity conditions.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
6.9
作者：
Ardie SW;Xie L;Takahashi R;Liu S;Takano T
通讯作者：
Takano T
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
4.1
作者：
Goyal, K;Walton, LJ;Tunnacliffe, A
通讯作者：
Tunnacliffe, A