Lead, Cadmium and Zinc Phytotoxicity Alter DNA Methylation Levels to Confer Heavy Metal Tolerance in Wheat
Lead, Cadmium and Zinc Phytotoxicity Alter DNA Methylation Levels to Confer Heavy Metal Tolerance in Wheat
复制标题
铅、镉和锌的植物毒性改变 DNA 甲基化水平，赋予小麦重金属耐受性
DOI：
10.3390/ijms20194676
复制
发表时间：
2019-09
影响因子：
5.6
通讯作者：
Liu Liangyu
中科院分区：
文献类型：
--
作者：
Shafiq Sarfraz;Zeb Qudsia;Ali Asim;Sajjad Yasar;Nazir Rashid;Widemann Emilie;Liu Liangyu
作为一种主食，小麦（Triticum aestivum）在营养上满足了人类健康的所有需求，也是人类和动物摄入污染物的食物链中的重要环节。因此，土壤中铅（Pb）、镉（Cd）等重金属的存在不仅导致小麦减产，而且对人畜健康构成潜在威胁。然而，DNA甲基化与小麦耐重金属胁迫之间的关系尚未得到研究。本研究根据小麦对铅胁迫的表型反应筛选出8个高产小麦品种。其中，Pirsabak 2004和Fakhar-e-sarhad分别被鉴定为抗铅和敏感品种。此外，Pirsabak 2004和Fakhar-e-sarhad品种也被发现分别对Cd和Zn胁迫具有抗性和敏感性。抗氧化活性下降，在Fakhar-e-sarhad与对照相比，在响应铅/镉/锌胁迫，但Fakhar-e-sarhad和Pirsabak 2004年积累了类似的铅，镉和锌在他们的根水平。重金属ATP酶2（TaHMA 2）和ATP结合盒（TaABCC 2/3/4）的金属解毒转运蛋白的表达显着上调Pirsabak 2004相比，Fakhar-e-sarhad和未处理的控制响应铅，镉和锌金属胁迫。与金属解毒转运蛋白的上调一致，在Pirsabak 2004中，与Fakhar-e-sarhad和未处理的对照相比，在这些转运蛋白的启动子区域也发现CG DNA低甲基化，这表明DNA甲基化调节金属解毒转运蛋白的表达，从而赋予小麦对金属毒性的抗性。本研究建议农民在重金属污染的土壤中种植Pirsabak 2004品种，并强调DNA甲基化与小麦的金属胁迫耐受性有关。
Being a staple food, wheat (Triticum aestivum) nutritionally fulfills all requirements of human health and also serves as a significant link in the food chain for the ingestion of pollutants by humans and animals. Therefore, the presence of the heavy metals such as lead (Pb) and cadmium (Cd) in soil is not only responsible for the reduction of wheat crop yield but also the potential threat for human and animal health. However, the link between DNA methylation and heavy metal stress tolerance in wheat has not been investigated yet. In this study, eight high yielding wheat varieties were screened based on their phenotype in response to Pb stress. Out of these, Pirsabak 2004 and Fakhar-e-sarhad were identified as Pb resistant and sensitive varieties, respectively. In addition, Pirsabak 2004 and Fakhar-e-sarhad varieties were also found resistant and sensitive to Cd and Zinc (Zn) stress, respectively. Antioxidant activity was decreased in Fakhar-e-sarhad compared with control in response to Pb/Cd/Zn stresses, but Fakhar-e-sarhad and Pirsabak 2004 accumulated similar levels of Pb, Cd and Zn in their roots. The expression of Heavy Metal ATPase 2 (TaHMA2) and ATP-Binding Cassette (TaABCC2/3/4) metal detoxification transporters are significantly upregulated in Pirsabak 2004 compared with Fakhar-e-sarhad and non-treated controls in response to Pb, Cd and Zn metal stresses. Consistent with upregulation of metal detoxification transporters, CG DNA hypomethylation was also found at the promoter region of these transporters in Pirsabak 2004 compared with Fakhar-e-sarhad and non-treated control, which indicates that DNA methylation regulates the expression of metal detoxification transporters to confer resistance against metal toxicity in wheat. This study recommends the farmers to cultivate Pirsabak 2004 variety in metal contaminated soils and also highlights that DNA methylation is associated with metal stress tolerance in wheat.
登录
查看更多内容
影响因子：
7.9
作者：
Vilahur, Nadia;Vahter, Marie;Broberg, Karin
通讯作者：
Broberg, Karin
实验方案：植物中 RNA 指导的 DNA 甲基化分析的初学者指南。
影响因子：
5.1
作者：
Zhang H;Tang K;Wang B;Duan CG;Lang Z;Zhu JK
通讯作者：
Zhu JK
影响因子：
--
作者：
Kailash Gulshan;D. L. Mason;Guosheng Liu;P. A. Rea;A. Bachhawat;S. Michaelis
通讯作者：
Kailash Gulshan;D. L. Mason;Guosheng Liu;P. A. Rea;A. Bachhawat;S. Michaelis
影响因子：
2.4
作者：
Csiszar, J.;Lantos, E.;Erdei, L.
通讯作者：
Erdei, L.
DOI：
10.1007/978-94-007-5179-8_13
发表时间：
2013-01-01
期刊：
CADMIUM: FROM TOXICITY TO ESSENTIALITY
影响因子：
--
作者：
Andresen, Elisa;Kuepper, Hendrik
通讯作者：
Kuepper, Hendrik