Determination of Akt/PKB Signaling

Determination of Akt/PKB Signaling
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Akt/PKB 信号传导的测定

DOI:
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发表时间:
2004
影响因子:
--
通讯作者:
R. Pankov
R. Pankov
中科院分区:
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文献类型:
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作者:
R. Pankov

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This unit provides a basic protocol for assaying the level of activity, as well as the activation capacity and dynamics of inhibition of Akt/PKB in cultured cells. All these data can be obtained in a single nonradioactive experiment by standard techniques including immunoblotting and immunodetection with phosphospecific antibodies.
DOI: --
发表时间: 2001-07
影响因子: 4
作者:
Kenneth M. Yamada;Masaru Araki
通讯作者: Kenneth M. Yamada;Masaru Araki