Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapyin vivo
Luciferase activity as a marker of tumor burden and as an indicator of tumor response to antineoplastic therapyin vivo
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荧光素酶活性作为肿瘤负荷的标志物和作为体内肿瘤抗肿瘤治疗反应的指标
DOI：
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发表时间：
1994
影响因子：
4
通讯作者：
Lieping Chen
中科院分区：
文献类型：
--
作者：
Lurong Zhang;K. Hellström;Lieping Chen
编码萤火虫荧光素酶的基因已被用作研究基因功能的报告基因。荧光素酶催化其底物并随后产生发光。此外，它不存在于哺乳动物细胞中。因此，我们探索了其在体内监测肿瘤生长中的用途。将荧光素酶基因转染入两种小鼠肿瘤系，即cl 62黑色素瘤和M109肺癌，并通过快速荧光反应测定与细胞相关的荧光素酶活性。荧光素酶活性与体外培养的肿瘤细胞数呈良好的相关性。荧光素酶活性还与体内肿瘤负荷相关，以及与同基因C3 H/HeN小鼠实验肿瘤模型中过继性T细胞转移治疗的效果相关。该方法具有定量、快速、可靠的优点，可用于肿瘤负荷的检测和化疗效果的评价。
The gene encoding firefly luciferase has been used as a reporter gene for the study of gene function. The luciferase catalyzes its substrate and subsequently produces luminescence. In addition, it is not present in mammalian cells. We have therefore explored its use in monitoring the growth of tumorsin vivo. The luciferase gene was transfected into two murine tumor lines, i.e. cl62 melanoma and M109 lung carcinoma, and the luciferase activity associated with the cells was determined by a rapid chemiluminescent reaction. Luciferase activity was well-correlated with the number of tumor cellsin vitro. Luciferase activity also correlated with the tumor burdenin vivo, as well as with the effect of an adoptive T cell transfer therapy in the syngeneic C3H/HeN mice experimental tumor model. This assay offers the advantage of being quantitative, rapid, and reliable for the detection of tumor burden and for the evaluation of the effect of antineoplastic therapy.
将转基因细胞移植到大鼠大脑中：大肠杆菌β-半乳糖苷酶作为报告基因的特征。
DOI：
10.1016/0169-328x(89)90061-2
发表时间：
1989
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Shimohama,S;Rosenberg,MB;Fagan,AM;Wolff,JA;Short,MP;Breakefield,XO;Friedmann,T;Gage,FH
通讯作者：
Gage,FH
细菌 lacZ 基因作为检测肿瘤进展过程中微转移形成的高度敏感标记。
影响因子：
11.2
作者：
Lin,WC;Pretlow,TP;Pretlow2nd,TG;Culp,LA
通讯作者：
Culp,LA
表达不同水平的人类黑色素瘤相关抗原 p97 的转染小鼠黑色素瘤系。
DOI：
10.1093/jnci/81.6.445
发表时间：
1989
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Estin,CD;Stevenson,U;Kahn,M;Hellström,I;Hellström,KE
通讯作者：
Hellström,KE