The nanovirus U2 protein suppresses RNA silencing via three conserved cysteine residues.
The nanovirus U2 protein suppresses RNA silencing via three conserved cysteine residues.
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DOI：
10.1111/mpp.13394
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发表时间：
2024-01
影响因子：
4.9
通讯作者：
Yan, Fei
中科院分区：
文献类型：
--
作者：
Yan, Dankan;Han, Kelei;Lu, Yuwen;Peng, Jiejun;Rao, Shaofei;Wu, Guanwei;Liu, Yong;Chen, Jianping;Zheng, Hongying;Yan, Fei
纳米病毒具有多部分、环状、单链DNA基因组，并在豆类和其他作物中造成巨大的产量损失。目前还没有来自纳米病毒属成员的RNA沉默病毒抑制因子（VSR）的报道。在这里，我们证明了纳米病毒U2蛋白是VSR。紫云英矮缩病毒（MDV）的U2蛋白抑制由单链和双链RNA诱导的绿色荧光蛋白（GFP）基因的沉默以及GFP沉默信号的系统传播。电泳迁移率变动分析表明，U2蛋白能够结合双链21个核苷酸的小干扰RNA（siRNA）。MDV U2蛋白中43、79和82位的半胱氨酸残基对其核定位、自身相互作用和siRNA结合能力至关重要，并且对其VSR活性至关重要。此外，通过马铃薯X病毒载体表达的U2蛋白诱导更严重的坏死症状，本塞姆氏烟草叶片。其他纳米病毒的U2蛋白也充当VSR，并且三个保守的半胱氨酸残基对于其VSR活性是不可缺少的。纳米病毒U2蛋白被鉴定为RNA沉默的抑制因子。三个半胱氨酸残基对其核定位、自身相互作用和siRNA结合能力至关重要，并且对其VSR活性至关重要。
Nanoviruses have multipartite, circular, single‐stranded DNA genomes and cause huge production losses in legumes and other crops. No viral suppressor of RNA silencing (VSR) has yet been reported from a member of the genus Nanovirus. Here, we demonstrate that the nanovirus U2 protein is a VSR. The U2 protein of milk vetch dwarf virus (MDV) suppressed the silencing of the green fluorescent protein (GFP) gene induced by single‐stranded and double‐stranded RNA, and the systemic spread of the GFP silencing signal. An electrophoretic mobility shift assay showed that the U2 protein was able to bind double‐stranded 21‐nucleotide small interfering RNA (siRNA). The cysteine residues at positions 43, 79 and 82 in the MDV U2 protein are critical to its nuclear localization, self‐interaction and siRNA‐binding ability, and were essential for its VSR activity. In addition, expression of the U2 protein via a potato virus X vector induced more severe necrosis symptoms in Nicotiana benthamiana leaves. The U2 proteins of other nanoviruses also acted as VSRs, and the three conserved cysteine residues were indispensable for their VSR activity. The nanovirus U2 protein was identified as the suppressor of RNA silencing. Three cysteine residues were critical to its nuclear localization, self‐interaction and siRNA‐binding ability, and were essential for its VSR activity.
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DOI：
10.3390/v12101111
发表时间：
2020-09-30
期刊：
Viruses
影响因子：
--
作者：
Zhang CW;Liu Q;Zeng Q;Huang WT;Wang Q;Cheng YQ
通讯作者：
Cheng YQ
影响因子：
6.7
作者：
Kim H;Shimura H;Sueda K;Masuta C
通讯作者：
Masuta C
影响因子：
11.6
作者：
DONALD, RGK;JACKSON, AO
通讯作者：
JACKSON, AO
影响因子：
5.4
作者：
Dong, XL;van Wezel, R;Hong, YG
通讯作者：
Hong, YG
水稻矮缩病毒编码的一种非结构蛋白靶向细胞核和叶绿体并抑制局部 RNA 沉默。
影响因子：
9.1
作者：
Gao, Feng;Zhao, Shanshan;Li, Yi
通讯作者：
Li, Yi