Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.
Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.
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DOI：
10.1083/jcb.200706134
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发表时间：
2007-09-10
期刊：
影响因子：
--
通讯作者：
Bellini M
中科院分区：
文献类型：
--
作者：
Patel SB;Novikova N;Bellini M
In amphibian oocytes, most lateral loops of the lampbrush chromosomes correspond to active transcriptional sites for RNA polymerase II. We show that newly assembled small nuclear ribonucleoprotein (RNP [snRNP]) particles, which are formed upon cytoplasmic injection of fluorescently labeled spliceosomal small nuclear RNAs (snRNAs), target the nascent transcripts of the chromosomal loops. With this new targeting assay, we demonstrate that nonfunctional forms of U1 and U2 snRNAs still associate with the active transcriptional units. In particular, we find that their association with nascent RNP fibrils is independent of their base pairing with pre–messenger RNAs. Additionally, stem loop I of the U1 snRNA is identified as a discrete domain that is both necessary and sufficient for association with nascent transcripts. Finally, in oocytes deficient in splicing, the recruitment of U1, U4, and U5 snRNPs to transcriptional units is not affected. Collectively, these data indicate that the recruitment of snRNPs to nascent transcripts and the assembly of the spliceosome are uncoupled events.
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影响因子：
4.5
作者：
Lund, M;Kjems, J
通讯作者：
Kjems, J
影响因子：
4.8
作者：
Degot, S;Le Hir, H;Tomasetto, C
通讯作者：
Tomasetto, C
影响因子：
4.5
作者：
Du, H;Rosbash, M
通讯作者：
Rosbash, M
DOI：
10.1083/jcb.133.4.719
发表时间：
1996-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang S;Spector DL
通讯作者：
Spector DL
影响因子：
4.5
作者：
Dönmez, G;Hartmuth, K;Lührmann, R
通讯作者：
Lührmann, R