Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription.
Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription.
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DOI：
10.1083/jcb.133.4.719
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发表时间：
1996-05
期刊：
影响因子：
--
通讯作者：
Spector DL
中科院分区：
文献类型：
--
作者：
Huang S;Spector DL
我们已经研究了瞬时和稳定表达的新生RNA转录本的核定位，含有或缺乏内含子，以确定是否在核中的RNA转录本和前mRNA剪接因子的空间关联是随机的或功能显着的。我们的研究结果表明，新生RNA和剪接因子之间的关联在核内是内含子依赖的RNA瞬时或稳定表达时。此外，我们的数据表明剪接因子被募集到转录位点。前体和mRNA在这些位置的存在表明前体mRNA剪接发生在这些转录位点。此外，电子显微镜检查的高度活跃的转录位点揭示了一个颗粒状的外观非常相似，但功能不同，染色质间颗粒簇。我们的研究结果表明，细胞核是高度组织化和动态的转录和前mRNA剪接的功能。
We have examined the nuclear localization of transiently and stably expressed nascent RNA transcripts containing or lacking introns in order to determine if the spatial association of RNA transcripts and pre-mRNA splicing factors in nuclei is random or functionally significant. Our findings show that the association between nascent RNA and splicing factors in the nucleus is intron-dependent when the RNAs are either transiently or stably expressed. Furthermore, our data indicate that splicing factors are recruited to the transcription sites. The presence of both pre-and mRNA at these locations suggest that pre-mRNA splicing occurs at these sites of transcription. In addition, electron microscopic examination of the highly active transcription sites has revealed a granular appearance which closely resembles, but is functionally different from, interchromatin granule clusters. Our findings demonstrate that the nucleus is highly organized and dynamic with regard to the functions of the transcription and pre- mRNA splicing.
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影响因子：
56.9
作者：
CACERES, JF;STAMM, S;KRAINER, AR
通讯作者：
KRAINER, AR
DOI：
10.1083/jcb.124.3.249
发表时间：
1994-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
O'Keefe RT;Mayeda A;Sadowski CL;Krainer AR;Spector DL
通讯作者：
Spector DL
影响因子：
64.5
作者：
JIMENEZGARCIA, LF;SPECTOR, DL
通讯作者：
SPECTOR, DL
影响因子：
64.8
作者：
GUI, JF;LANE, WS;FU, XD
通讯作者：
FU, XD
DOI：
10.1073/pnas.78.5.2737
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LERNER, EA;LERNER, MR;STEITZ, JA
通讯作者：
STEITZ, JA