Amplification of epidermal growth factor receptor gene but no evidence of ras mutations in primary human esophageal cancers.
Amplification of epidermal growth factor receptor gene but no evidence of ras mutations in primary human esophageal cancers.
复制标题
表皮生长因子受体基因扩增，但没有证据表明原发性人食道癌中存在 ras 突变。
作者：
M. Hollstein;A. Smits;C. Galiana;H. Yamasaki;J. Bos;A. Mandard;C. Partensky;R. Montesano
我们分析了41例原发性食管鳞状细胞癌中与这种恶性肿瘤相关的原癌基因改变的存在。采用聚合酶链反应扩增的靶序列寡聚体杂交法检测25例肿瘤中ras基因突变。我们没有发现H-ras、K-ras和N-ras基因密码子12和61突变的证据，也没有发现K-ras和N-ras基因座密码子13突变的证据。食管癌中明显缺乏激活的ras癌基因，这可能是在许多其他类型的人类恶性肿瘤中发现的这些突变的存在的一个例外，并且与人类结直肠癌中40%的ras突变流行率形成鲜明对比。肿瘤DNA的Southern印迹杂交实验表明，在25例癌中有2例表皮生长因子受体基因（c-erbB）扩增。未检测到结构相关的c-erbB 2（neu）基因扩增。12例食管癌中有3例表皮生长因子受体RNA表达水平明显高于正常食管粘膜组织。我们的研究结果表明，表皮生长因子受体基因的转录增强与一些食管癌的发展。
Primary esophageal squamous cell carcinomas from 41 patients were analyzed for the presence of proto-oncogene alterations associated with this malignancy. The occurrence of activating ras gene mutations in 25 tumors was determined using oligomer hybridization of target sequences amplified by polymerase chain reaction. We found no evidence for mutations in codons 12 and 61 of the H-ras, K-ras, and N-ras genes, nor in codon 13 of the K-ras and N-ras loci in any of these tumors. The apparent absence of activated ras oncogene in esophageal cancers represents a possible exception to the presence of these mutations found consistently in numerous other types of human malignancies, and is in striking contrast to the 40% prevalence of ras mutations in human colorectal cancers. Southern blot hybridization experiments with DNAs from tumors demonstrated amplification of the epidermal growth factor receptor gene (c-erbB) in two of 25 carcinomas. No amplification of the structurally related c-erbB2 (neu) gene was detected. In three out of 12 carcinomas, the level of epidermal growth factor receptor RNA was significantly higher than in normal esophageal mucosal tissue. Our results suggest that enhanced transcription of the epidermal growth factor receptor gene is associated with the development of some esophageal cancers.
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影响因子：
56.9
作者：
BISHOP, JM
通讯作者：
BISHOP, JM
DOI：
10.1016/0165-1110(87)90021-2
发表时间：
1987
期刊：
Mutation research
影响因子：
--
作者：
Guerrero,I;Pellicer,A
通讯作者：
Pellicer,A
DOI：
10.1073/pnas.84.19.6899
发表时间：
1987-10-01
影响因子：
11.1
作者：
WONG, AJ;BIGNER, SH;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
人类恶性疾病中原癌基因扩增的特异性。
DOI：
--
发表时间：
1987
期刊：
Molecular biology & medicine
影响因子：
--
作者：
Masuda,H;Battifora,H;Yokota,J;Meltzer,S;Cline,MJ
通讯作者：
Cline,MJ