Identification and characterization of microRNAs from peanut (Arachis hypogaea L.) by high-throughput sequencing.
Identification and characterization of microRNAs from peanut (Arachis hypogaea L.) by high-throughput sequencing.
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通过高通量测序鉴定和表征花生 (Arachishypogaea L.) 的 microRNA
DOI：
10.1371/journal.pone.0027530
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Yu S
中科院分区：
文献类型：
--
作者：
Chi X;Yang Q;Chen X;Wang J;Pan L;Chen M;Yang Z;He Y;Liang X;Yu S
研究背景MicroRNAs（miRNAs）是一类长度约为21 nt的非编码RNA，通过核酸内切酶切割或翻译抑制等方式调控植物基因的转录后表达。miRNAs在许多发育和生理过程中发挥重要作用，其中许多在物种间是保守的。已经在一些模式植物中对miRNAs进行了广泛的研究;然而，对花生（Arachis hypogaea L.）中这些调控RNA的多样性知之甚少，世界上最重要的油料作物之一。结果构建了花生小RNA文库，并进行了序列分析。除了来自33个家族的126个已知miRNAs外，还鉴定了25个新的花生miRNAs。发现了四种新的miRNA的miRNA* 序列，为miRNA的存在提供了额外的证据。20种新的miRNAs被认为是物种特异性的，因为没有发现其他植物物种的同源物。采用qRT-PCR方法分析了7种miRNAs在不同组织和不同发育阶段种子中的表达，其中一些miRNAs表现出组织和/或生长阶段特异性表达。此外，在序列同源性搜索的基础上，预测这些推定的miRNAs的潜在靶标。结论通过对小RNA文库的深度测序，我们鉴定出大量的miRNAs及其相关靶基因。本研究对花生中miRNAs的鉴定和特征分析，为进一步研究花生miRNAs的调控机制奠定了基础，有助于更好地理解miRNAs在花生中的重要作用。
Background MicroRNAs (miRNAs) are noncoding RNAs of approximately 21 nt that regulate gene expression in plants post-transcriptionally by endonucleolytic cleavage or translational inhibition. miRNAs play essential roles in numerous developmental and physiological processes and many of them are conserved across species. Extensive studies of miRNAs have been done in a few model plants; however, less is known about the diversity of these regulatory RNAs in peanut (Arachis hypogaea L.), one of the most important oilseed crops cultivated worldwide. Results A library of small RNA from peanut was constructed for deep sequencing. In addition to 126 known miRNAs from 33 families, 25 novel peanut miRNAs were identified. The miRNA* sequences of four novel miRNAs were discovered, providing additional evidence for the existence of miRNAs. Twenty of the novel miRNAs were considered to be species-specific because no homolog has been found for other plant species. qRT-PCR was used to analyze the expression of seven miRNAs in different tissues and in seed at different developmental stages and some showed tissue- and/or growth stage-specific expression. Furthermore, potential targets of these putative miRNAs were predicted on the basis of the sequence homology search. Conclusions We have identified large numbers of miRNAs and their related target genes through deep sequencing of a small RNA library. This study of the identification and characterization of miRNAs in peanut can initiate further study on peanut miRNA regulation mechanisms, and help toward a greater understanding of the important roles of miRNAs in peanut.
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影响因子：
3.7
作者：
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影响因子：
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影响因子：
14.9
作者：
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