Knockdown of Unconventional Myosin ID Expression Induced Morphological Change in Oligodendrocytes.
Knockdown of Unconventional Myosin ID Expression Induced Morphological Change in Oligodendrocytes.
复制标题
DOI：
10.1177/1759091416669609
复制
发表时间：
2016-10
期刊：
影响因子：
4.7
通讯作者：
Yamaguchi Y
中科院分区：
文献类型：
--
作者：
Yamazaki R;Ishibashi T;Baba H;Yamaguchi Y
髓鞘是一种特殊的多层结构，参与神经系统的多种功能。在中枢神经系统中，少突胶质细胞（OL）产生髓鞘并具有独特的形态。OL具有与细胞骨架组织相关的动态膜分选系统，其有助于髓鞘的产生。最近，有报道称肌动蛋白丝的组装和拆卸对于髓鞘形成至关重要。然而，在髓鞘形成过程中与肌动蛋白丝结合的伙伴肌球蛋白分子尚未被确定。一种候选肌球蛋白是非常规肌球蛋白ID（Myo 1d），其分布于整个中枢神经系统髓鞘中;然而，其功能仍不清楚。我们在这里报告，Myo 1d在OL分化的后期阶段，连同髓鞘蛋白脂质蛋白（PLP）的表达。此外，Myo 1d还分布于培养的OL的髓鞘样膜前缘，主要与肌动蛋白丝、2′，3 ′-环核苷酸磷酸二酯酶共定位，部分与PLP共定位。特异性siRNA敲低Myo 1d可引起细胞形态学改变，如突起回缩、髓鞘样膜变性，最终导致细胞凋亡。此外，Myo 1d的siRNA的损失导致细胞内PLP转运的损害。总之，这些结果表明，Myo 1d可能有助于膜动力学的髓鞘形成和维持过程中的髓鞘膜蛋白的包裹或运输。
Myelin is a special multilamellar structure involved in various functions in the nervous system. In the central nervous system, the oligodendrocyte (OL) produces myelin and has a unique morphology. OLs have a dynamic membrane sorting system associated with cytoskeletal organization, which aids in the production of myelin. Recently, it was reported that the assembly and disassembly of actin filaments is crucial for myelination. However, the partner myosin molecule which associates with actin filaments during the myelination process has not yet been identified. One candidate myosin is unconventional myosin ID (Myo1d) which is distributed throughout central nervous system myelin; however, its function is still unclear. We report here that Myo1d is expressed during later stages of OL differentiation, together with myelin proteolipid protein (PLP). In addition, Myo1d is distributed at the leading edge of the myelin-like membrane in cultured OL, colocalizing mainly with actin filaments, 2′,3′-cyclic nucleotide phosphodiesterase and partially with PLP. Myo1d-knockdown with specific siRNA induces significant morphological changes such as the retraction of processes and degeneration of myelin-like membrane, and finally apoptosis. Furthermore, loss of Myo1d by siRNA results in the impairment of intracellular PLP transport. Together, these results suggest that Myo1d may contribute to membrane dynamics either in wrapping or transporting of myelin membrane proteins during formation and maintenance of myelin.
登录
查看更多内容
影响因子：
2.9
作者：
Benesh AE;Fleming JT;Chiang C;Carter BD;Tyska MJ
通讯作者：
Tyska MJ
影响因子：
3.3
作者：
Hokanson, David E.;Laakso, Joseph M.;Ostap, E. Michael
通讯作者：
Ostap, E. Michael
影响因子：
4.8
作者：
Patino-Lopez, Genaro;Aravind, L.;Shaw, Stephen
通讯作者：
Shaw, Stephen
影响因子：
11.8
作者：
Nawaz S;Sánchez P;Schmitt S;Snaidero N;Mitkovski M;Velte C;Brückner BR;Alexopoulos I;Czopka T;Jung SY;Rhee JS;Janshoff A;Witke W;Schaap IAT;Lyons DA;Simons M
通讯作者：
Simons M
影响因子：
64.8
作者：
Hozumi, S;Maeda, R;Matsuno, K
通讯作者：
Matsuno, K