Expression and localization of myosin-1d in the developing nervous system.
Expression and localization of myosin-1d in the developing nervous system.
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DOI：
10.1016/j.brainres.2011.12.054
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发表时间：
2012-02-27
期刊：
影响因子：
2.9
通讯作者：
Tyska MJ
中科院分区：
文献类型：
--
作者：
Benesh AE;Fleming JT;Chiang C;Carter BD;Tyska MJ
肌球蛋白-1d是一种以肌动蛋白为基础的单体马达，广泛存在于各种组织中，但在神经系统中高度表达。以前的微阵列研究表明，肌球蛋白-1d被发现在少突胶质细胞的转录上调，在这些细胞的成熟。在蛋白质组学研究中，肌球蛋白-1d也被鉴定为含髓鞘亚细胞组分的组分，MYO 1D的突变与自闭症有关。尽管这些以前的研究的潜在影响，有很少的信息肌球蛋白-1d在发育中的神经系统的表达和定位。因此，我们分析了肌球蛋白-1d在出生后发育的外周和中枢神经系统的表达模式。在小鼠坐骨神经中，肌球蛋白-1 d沿着轴突和髓鞘隔室表达。分析小鼠小脑髓鞘形成前在第3天揭示肌球蛋白-1d存在于浦肯野细胞层，颗粒细胞层，和小脑核的区域。在髓鞘形成开始时，肌球蛋白-1d富集沿着轴突束扩展，同时仍然存在于浦肯野和颗粒细胞层中。然而，肌球蛋白-1d在少突胶质细胞祖细胞在早期和晚期时间点检测不到。我们还表明，肌球蛋白-1d相互作用，并与脂肪酰化酶，在整个神经系统的脂肪酸合成中起着关键作用的酶共同表达。总之，这些研究为理解肌球蛋白-1d在神经发育和神经系统疾病中的作用提供了基础。
Myosin-1d is a monomeric actin-based motor found in a wide range of tissues, but highly expressed in the nervous system. Previous microarray studies suggest that myosin-1d is found in oligodendrocytes where transcripts are upregulated during the maturation of these cells. Myosin-1d was also identified as a component of myelin-containing subcellular fractions in proteomic studies and mutations in MYO1D have been linked to autism. Despite the potential implications of these previous studies, there is little information on the expression and localization of myosin-1d in the developing nervous system. Therefore, we analyzed myosin-1d expression patterns in the peripheral and central nervous systems during postnatal development. In mouse sciatic nerve, myosin-1d is expressed along the axon and in the ensheathing myelin compartment. Analysis of mouse cerebellum prior to myelination at day 3 reveal myosin-1d is present in the Purkinje cell layer, granule cell layer, and region of the cerebellar nuclei. Upon the onset of myelination, myosin-1d enrichment expands along axonal tracts, while still present in the Purkinje and granule cell layers. However, myosin-1d was undetectable in oligodendrocyte progenitor cells at early and late time points. We also show that myosin-1d interacts and is co-expressed with aspartoacylase, an enzyme that plays a key role in fatty acid synthesis throughout the nervous system. Together, these studies provide a foundation for understanding the role of myosin-1d in neurodevelopment and neurological disorders.
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影响因子：
2.9
作者：
BAIMBRIDGE, KG;MILLER, JJ
通讯作者：
MILLER, JJ
DOI：
10.1073/pnas.0607817104
发表时间：
2007-01-09
影响因子：
11.1
作者：
Bitto, Eduard;Bingman, Craig A.;Phillips, George N., Jr.
通讯作者：
Phillips, George N., Jr.
影响因子：
3.1
作者：
Baslow, MH;Suckow, RF;Hungund, BL
通讯作者：
Hungund, BL
影响因子：
30.8
作者：
KAUL, R;GAO, GP;MATALON, R
通讯作者：
MATALON, R
影响因子：
3.3
作者：
Hokanson, David E.;Laakso, Joseph M.;Ostap, E. Michael
通讯作者：
Ostap, E. Michael