α-MSH regulates interleukin-10 expression by human keratinocytes
α-MSH regulates interleukin-10 expression by human keratinocytes
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α-MSH 调节人角质细胞的白细胞介素 10 表达
DOI：
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发表时间：
1998
影响因子：
3
通讯作者：
E. Bandrés
中科院分区：
文献类型：
--
作者：
Pedro Redondo;J. García;I. Okroujnov;E. Bandrés
正常人角质形成细胞（HKC）具有合成α-MSH的能力。由于紫外线B辐射可显著诱导HKC产生α-MSH，因此已提出角质形成细胞衍生的α-MSH参与UV诱导的免疫抑制。α-MSH对单核细胞内IL-10的诱导作用是其抗肿瘤作用的主要机制。在本研究中，研究了HKC在α-MSH刺激后产生IL-10的能力。从人女性乳房切片的皮肤中获得HKC，并不处理或用0.01或0.1 μg/ml α-MSH处理不同时间。RT-PCR显示，HKC在基础条件下仍能表达IL-10 mRNA，α-MSH处理后表达增加。在α-MSH刺激培养物的上清液中仅检测到最低浓度的IL-10蛋白。据我们所知，这是第一次报道培养的HKC在α-MSH刺激后表达IL-10。需要进一步的研究来确定这些发现的生物学和治疗相关性。
Abstract Normal human keratinocytes (HKC) are able to synthesize α-MSH. Because the production of α-MSH by HKC is induced significantly by ultraviolet B radiation, the involvement of keratinocyte-derived α-MSH in UV-induced immunosuppression has been suggested. The induction of the antiinflammatory cytokine IL-10 in monocytes is a major mechanism in the antiinflammatory actions of α-MSH. In the present study, HKC were investigated for their ability to produce IL-10 after α-MSH stimulation. HKC were obtained from the skin of human female breast sections and either left untreated or treated with 0.01 or 0.1 μg/ml α-MSH for different times. Using RT-PCR, HKC were shown to express IL-10 mRNA even under basal conditions, and treatment with α-MSH increased expression. Only minimal concentrations of IL-10 protein were detected in supernatants from the α-MSH-stimulated cultures. To the best of our knowledge this is the first report of IL-10 expression by cultured HKC after α-MSH stimulation. Further studies are needed to determine the biological and therapeutic relevance of these findings.
DOI：
10.1016/0022-202x(89)90177-2
发表时间：
1989-05
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
J. L. Bolognia;Marilyn Murray;John M. Pawelek
通讯作者：
J. L. Bolognia;Marilyn Murray;John M. Pawelek
粘附分子、趋化因子和细胞因子在炎症和肿瘤性皮肤病中的作用 - 1990 年更新。
DOI：
10.1111/1523-1747.ep12876134
发表时间：
1990
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Nickoloff,BJ;Griffiths,CE;Barker,JN
通讯作者：
Barker,JN
紫外线照射的角质形成细胞上清液对迟发型超敏反应的全身抑制。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Rivas,JM;Ullrich,SE
通讯作者：
Ullrich,SE