AQP5 Is a Novel Prognostic Biomarker in Pancreatic Adenocarcinoma.
AQP5 Is a Novel Prognostic Biomarker in Pancreatic Adenocarcinoma.
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DOI：
10.3389/fonc.2022.890193
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发表时间：
2022
影响因子：
4.7
通讯作者：
Wei, Di
中科院分区：
文献类型：
--
作者：
Chen, Guo;Song, Haiyang;Yang, Zelong;Du, Tianshu;Zheng, Yu;Lu, Zifan;Zhang, Kunpeng;Wei, Di
胰腺癌是一种高度恶性的肿瘤，预后差。寻找有效的分子标记物对诊断和治疗具有重要意义。水通道蛋白（Aquaporins，AQP）是一类水通道蛋白，具有多种生物学特性，在人类肿瘤的发生发展过程中起着重要的调控作用。然而，水通道蛋白-5（AQP 5）的表达与预后和肿瘤浸润淋巴细胞在PAAD中的关系尚未报道。使用GEPIA、UALCAN、HAP、METHSURV和UCSC数据库分析PAAD中AQP 5 mRNA表达、甲基化和蛋白表达数据。采用免疫组化和Western印迹法检测PAAD患者和来自我们队列的细胞系中AQP 5的表达。LinkedOmics数据库用于研究与AQP 5表达相关的信号通路。TIMER和TISIDB用于分析AQP 5、肿瘤浸润免疫细胞和免疫调节剂之间的相关性。使用TCGA和Kaplan-Meier Plotter数据库分析生存率。在这项研究中，我们研究了AQP 5在PAAD中的表达，并确定AQP 5的表达是否是PAAD的强有力的预后生物标志物。我们检索并分析了公共癌症数据库（GEO，TCGA，HAP，UALCAN，GEPIA等）。结论：PAAD中AQP 5表达水平上调。Kaplan-Meier曲线分析显示AQP 5高表达与预后不良呈正相关。使用TIMER和TISIDB，我们发现AQP 5的表达与不同的肿瘤浸润免疫细胞相关，尤其是巨噬细胞。我们发现AQP 5启动子区的低甲基化是其在PAAD中高表达的原因。AQP 5可作为预测PAAD预后和免疫浸润的新生物标志物。
Pancreatic adenocarcinoma (PAAD) is a highly malignant tumor with a poor prognosis. The identification of effective molecular markers is of great significance for diagnosis and treatment. Aquaporins (AQPs) are a family of water channel proteins that exhibit several properties and play regulatory roles in human carcinogenesis. However, the association between Aquaporin-5 (AQP5) expression and prognosis and tumor-infiltrating lymphocytes in PAAD has not been reported. AQP5 mRNA expression, methylation, and protein expression data in PAAD were analyzed using GEPIA, UALCAN, HAP, METHSURV, and UCSC databases. AQP5 expression in PAAD patients and cell lines from our cohort was examined using immunohistochemistry and Western blotting. The LinkedOmics database was used to study signaling pathways related to AQP5 expression. TIMER and TISIDB were used to analyze correlations among AQP5, tumor-infiltrating immune cells, and immunomodulators. Survival was analyzed using TCGA and Kaplan–Meier Plotter databases. In this study, we investigated AQP5 expression in PAAD and determined whether the expression of AQP5 is a strong prognostic biomarker for PAAD. We searched and analyzed public cancer databases (GEO, TCGA, HAP, UALCAN, GEPIA, etc.) to conclude that AQP5 expression levels were upregulated in PAAD. Kaplan–Meier curve analysis showed that high AQP5 expression positively correlated with poor prognosis. Using TIMER and TISIDB, we found that the expression of AQP5 was associated with different tumor-infiltrating immune cells, especially macrophages. We found that hypomethylation of the AQP5 promoter region was responsible for its high expression in PAAD. AQP5 can serve as a novel biomarker to predict prognosis and immune infiltration in PAAD.
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DOI：
10.1016/j.bbrc.2017.06.046
发表时间：
2017-08-19
影响因子：
3.1
作者：
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影响因子：
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