Bioactivating a bone substitute accelerates graft incorporation in a murine model of vertical ridge augmentation.
Bioactivating a bone substitute accelerates graft incorporation in a murine model of vertical ridge augmentation.
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生物激活骨替代物可加速垂直牙槽嵴增高小鼠模型中的移植物掺入
DOI：
10.1016/j.dental.2020.06.003
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Helms JA
中科院分区：
文献类型：
--
作者：
Chen J;Yuan X;Li Z;Bahat DJ;Helms JA
与自体骨移植物相比，同种异体骨移植物整合缓慢，这可能对临床结果产生不利影响。在这里，我们的目标是了解移植物掺入的分子机制，然后测试临床可行的方法来加速这一过程。野生型和转基因Wnt“报告基因”小鼠用于垂直嵴扩增程序。手术包括隧道手术，以提高上颌骨无牙嵴骨膜，然后插入骨移植。显微计算机断层扫描成像和分子/细胞分析用于随时间跟踪骨移植物。评价硬化蛋白缺失小鼠和携带活化形式的β-连环蛋白的小鼠以了解升高的Wnt信号传导如何影响无牙嵴高度，并且基于这些数据，采用仿生策略将联合收割机骨移植颗粒与重组WNT蛋白制剂组合。此后，评价移植物掺入率。骨钙素激活骨膜的骨祖细胞增殖。如果存在移植颗粒，则骨祖细胞附着于基质并产生新骨，增加无牙颌嵴高度。单独的移植物颗粒不刺激骨祖细胞增殖。基于Wnt信号放大的小鼠中较厚的无牙嵴，采取了一种策略，用WNT加载骨移植颗粒;这种组合足以加速移植物掺入的初始步骤。WNT蛋白治疗剂的局部递送具有加速移植物结合的潜力，并且因此缩短移植物可以支持牙种植体的时间。
Compared to autologous bone grafts, allogeneic bone grafts integrate slowly, which can adversely affect clinical outcomes. Here, our goal was to understand the molecular mechanisms underlying graft incorporation, and then test clinically feasible methods to accelerate this process. Wild-type and transgenic Wnt “reporter” mice were used in a vertical ridge augmentation procedure. The surgery consisted of tunneling procedure to elevate the maxillary edentulous ridge periosteum, followed by the insertion of bone graft. Micro-computed tomographic imaging, and molecular/cellular analyses were used to follow the bone graft over time. Sclerostin null mice, and mice carrying an activated form of β-catenin were evaluated to understand how elevated Wnt signaling impacted edentulous ridge height and based on these data, a biomimetic strategy was employed to combine bone graft particles with a formulation of recombinant WNT protein. Thereafter, the rate of graft incorporation was evaluated. Tunneling activated osteoprogenitor cell proliferation from the periosteum. If graft particles were present, then osteoprogenitor cells attached to the matrix and gave rise to new bone that augmented edentulous ridge height. Graft particles alone did not stimulate osteoprogenitor cell proliferation. Based on the thicker edentulous ridges in mice with amplified Wnt signaling, a strategy was undertaken to load bone graft particles with WNT; this combination was sufficient to accelerate the initial step of graft incorporation. Local delivery of a WNT protein therapeutic has the potential to accelerate graft incorporation, and thus shorten the time to when the graft can support a dental implant.
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影响因子：
2.5
作者：
Boyce RW;Brown D;Felx M;Mellal N;Locher K;Pyrah I;Ominsky MS;Taylor S
通讯作者：
Taylor S
影响因子：
4.6
作者：
KRAJICEK, DD;DOONER, J;PORTER, K
通讯作者：
PORTER, K
影响因子：
4.8
作者：
Filali, M;Cheng, NL;Engelhardt, JF
通讯作者：
Engelhardt, JF
影响因子：
6.2
作者：
Genant, Harry K.;Engelke, Klaus;Libanati, Cesar
通讯作者：
Libanati, Cesar
DOI：
10.1002/jbm.b.31529
发表时间：
2010-02-01
影响因子：
3.4
作者：
Figueiredo, Margarida;Henriques, Jose;Figueiredo, Helena
通讯作者：
Figueiredo, Helena