Transcriptomic analysis of differentially expressed genes in an orange-pericarp mutant and wild type in pummelo (Citrus grandis).
Transcriptomic analysis of differentially expressed genes in an orange-pericarp mutant and wild type in pummelo (Citrus grandis).
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柚子果皮突变体和野生型差异表达基因的转录组分析
DOI：
10.1186/s12870-015-0435-3
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发表时间：
2015-02-12
影响因子：
5.3
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Guo F;Yu H;Xu Q;Deng X
水果的外部颜色是一个至关重要的品质特征，大多数柑橘类水果的外部颜色是由于类胡萝卜素的积累造成的。由于缺乏突变体，类胡萝卜素生物合成和在果皮中积累的分子调控受到限制。在这项工作中，使用显示果皮色素沉着改变的橙色果皮突变体（MT）来鉴定可能与果皮中类胡萝卜素积累调节相关的基因。高效液相色谱 (HPLC) 分析显示，MT 果实的果皮中 β-胡萝卜素含量比野生型 (WT) 增加了 10.5 倍。实时定量PCR (qRT-PCR) 分析显示，MT 中所有下游胡萝卜素基因的表达均低于 WT，表明下调对于 MT 果皮中 β-胡萝卜素的增加至关重要。转录组的RNA-seq分析揭示了MT基因表达水平的广泛变化，有168个基因下调，135个基因上调。基因本体（GO）和KEGG途径分析表明，突变体中七个可靠的代谢途径发生了改变，包括碳代谢、淀粉和蔗糖代谢以及氨基酸的生物合成。通过 MT 果皮的 qRT-PCR 分析证实了与受影响的代谢途径相对应的转录因子和基因可能参与类胡萝卜素调节。这项研究提供了柚子果皮颜色不同的新型突变体基因表达变化的整体图景。对差异表达基因 (DEG) 的解释揭示了对 MT 果皮中 β-胡萝卜素积累的分子调控的新见解。本文的在线版本 (doi:10.1186/s12870-015-0435-3) 包含补充材料，可供授权用户使用。
The external colour of fruit is a crucial quality feature, and the external coloration of most citrus fruits is due to the accumulation of carotenoids. The molecular regulation of carotenoid biosynthesis and accumulation in pericarp is limited due to the lack of mutant. In this work, an orange-pericarp mutant (MT) which showed altered pigmentation in the pericarp was used to identify genes potentially related to the regulation of carotenoid accumulation in the pericarp. High Performance Liquid Chromatography (HPLC) analysis revealed that the pericarp from MT fruits had a 10.5-fold increase of β-carotene content over that of the Wild Type (WT). Quantitative real-time PCR (qRT-PCR) analysis showed that the expression of all downstream carotenogenic genes was lower in MT than in WT, suggesting that down-regulation is critical for the β-carotene increase in the MT pericarp. RNA-seq analysis of the transcriptome revealed extensive changes in the MT gene expression level, with 168 genes down-regulated and 135 genes up-regulated. Gene ontology (GO) and KEGG pathway analyses indicated seven reliable metabolic pathways are altered in the mutant, including carbon metabolism, starch and sucrose metabolism and biosynthesis of amino acids. The transcription factors and genes corresponding to effected metabolic pathways may involved in the carotenoid regulation was confirmed by the qRT-PCR analysis in the MT pericarp. This study has provided a global picture of the gene expression changes in a novel mutant with distinct color in the fruit pericarp of pummelo. Interpretation of differentially expressed genes (DEGs) revealed new insight into the molecular regulation of β-carotene accumulation in the MT pericarp. The online version of this article (doi:10.1186/s12870-015-0435-3) contains supplementary material, which is available to authorized users.
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影响因子：
6.1
作者：
Lee, HS
通讯作者：
Lee, HS
影响因子：
2.1
作者：
Ma, Juanjuan;Li, Jing;Han, Yuepeng
通讯作者：
Han, Yuepeng
DOI：
10.1111/j.1365-2621.1957.tb16983.x
发表时间：
1957-01-01
期刊：
FOOD RES
影响因子：
--
作者：
CURL, A. LAURENCE;BAILEY, GLEN F.
通讯作者：
BAILEY, GLEN F.
影响因子：
2.4
作者：
Licciardello, Concetta;Russo, Maria Patrizia;Recupero, Reforgiato Giuseppe
通讯作者：
Recupero, Reforgiato Giuseppe
影响因子：
5.3
作者：
Diretto, Gianfranco;Welsch, Ralf;Tavazza, Raffaela;Mourgues, Fabienne;Pizzichini, Daniele;Beyer, Peter;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni