The prognostic value of EGFR overexpression and amplification in Esophageal squamous cell Carcinoma.
The prognostic value of EGFR overexpression and amplification in Esophageal squamous cell Carcinoma.
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DOI：
10.1186/s12885-015-1393-8
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发表时间：
2015-05-08
期刊：
影响因子：
3.8
通讯作者：
Tan L
中科院分区：
文献类型：
--
作者：
Jiang D;Li X;Wang H;Shi Y;Xu C;Lu S;Huang J;Xu Y;Zeng H;Su J;Hou Y;Tan L
鉴于EGFR在癌细胞生物学中的突出作用和大量ESCC的改变，确定与患者预后相关的EGFR分子特征非常重要。因此，我们分析了上皮生长因子受体（EGFR）在中国食管鳞状细胞癌（ESCC）中的蛋白表达和基因拷贝变异，并探讨其与肿瘤各种特征和患者生存的可能关联。用96个ESCC组成的组织芯片切片，用免疫组化（IHC）检测EGFR表达，用荧光原位杂交（FISH）检测EGFR基因扩增。IHC的结果用六种不同的评分系统进行评估。采用卡方检验和Kaplan-Meier分析评价临床特征与生存率的相关性。根据评分系统1，EGFR过表达与晚期淋巴结受累（P = 0.046）、患者疾病特异性自由生存（DFS） （P = 0.006）和总生存（OS） （P = 0.007）显著相关。其他5种评分系统均无此相关性（P < 0.05）。EGFR扩增与淋巴结转移相关（P = 0.028），但与DFS和OS无关，直到20个月。通过评分系统1评估EGFR - IHC过表达可能适合用于预测ESCC患者的生存。EGFR基因扩增在20个月后显示延迟预后信息。本文的在线版本（doi:10.1186/s12885-015-1393-8）包含补充材料，仅供授权用户使用。
In view of the prominent role in cancer cell biology and alteration in substantial numbers of ESCC, defining EGFR molecular characteristics relevant to patient prognosis is of great importance. Therefore, we analyzed the protein expression and gene copy variation of the epithelial growth factor receptor (EGFR) in Chinese esophageal squamous cell carcinoma (ESCC) and explored the possible associations with various features of the tumors and survival of the patients. Sections were made from tissue microarray composed of 96 ESCC, and examined for EGFR expression by means of immunohistochemistry (IHC) and for EGFR gene amplification by means of fluorescence in situ hybridization (FISH). The results of IHC were evaluated with six different reported scoring systems. Correlation with clinical features and survival was evaluated using chi-square test and Kaplan–Meier analysis. EGFR overexpression according to scoring system 1 significantly correlated with advanced lymph node involvement (P = 0.046), patient disease specific free survival (DFS) (P = 0.006) and overall survival (OS) (P = 0.007). No such association was observed using other 5 scoring systems (P > 0.05 ). EGFR amplification was associated with lymph node metastasis (P = 0.028), but not correlated with DFS and OS until 20 months. EGFR IHC overexpression evaluated by scoring system 1 might be suitable to be used in predicting patients survival in ESCC. EGFR gene amplification showed delayed prognostic information after 20 months. The online version of this article (doi:10.1186/s12885-015-1393-8) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
Gonzaga IM;Soares-Lima SC;de Santos PT;Blanco TC;de Reis BS;Quintella DC;de Oliveira IM;de Faria PA;Kruel CD;Andreollo NA;de Simão TA;Pinto LF
通讯作者：
Pinto LF
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影响因子：
2.6
作者：
Shi Y;He D;Hou Y;Hu Q;Xu C;Liu Y;Jiang D;Su J;Zeng H;Tan Y
通讯作者：
Tan Y
DOI：
10.1097/coc.0b013e318201a126
发表时间：
2012-02-01
影响因子：
2.6
作者：
Dipetrillo, Thomas;Suntharalingam, Mohan;Safran, Howard
通讯作者：
Safran, Howard
影响因子：
51.1
作者：
Pirker, Robert;Pereira, Jose R.;O'Byrne, Kenneth J.
通讯作者：
O'Byrne, Kenneth J.
亚洲晚期非小细胞腺癌组织学肺癌患者 EGFR 突变的前瞻性分子流行病学研究 (PIONEER)。
DOI：
10.1097/jto.0000000000000033
发表时间：
2014-02
期刊：
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
影响因子：
--
作者：
Shi Y;Au JS;Thongprasert S;Srinivasan S;Tsai CM;Khoa MT;Heeroma K;Itoh Y;Cornelio G;Yang PC
通讯作者：
Yang PC