An alternative high output tissue microarray technique.
An alternative high output tissue microarray technique.
复制标题
另一种高输出组织微阵列技术
DOI：
10.1186/1746-1596-8-9
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发表时间：
2013-01-21
影响因子：
2.6
通讯作者：
Tan Y
中科院分区：
文献类型：
--
作者：
Shi Y;He D;Hou Y;Hu Q;Xu C;Liu Y;Jiang D;Su J;Zeng H;Tan Y
背景组织微阵列（TMA）是一种高通量的研究工具，它极大地方便和加速了原位组织分析。然而，由于传统供体块的厚度限制，其生产率受到限制。在这里，我们介绍了一种改进的高输出TMA方法，适用于更广泛的组织samples.MethodsIn这种方法，一个3.6厘米长，2.7厘米宽的收件人块88平方格（3毫米宽），首先使用几个商业仪器制备。然后用自制的刀片状刀从每个石蜡包埋的胃肠道间质瘤供体块制备2 mm宽和6 mm长的组织棒。将这些杆一个接一个地手动排列到60°C预软化的受体块的相应网格中，其中引导用钢针钻孔。结果制备的TMA具有良好的阵列结构、良好的组织形态和完整的蛋白质和DNA保存。从一个TMA块中可以很容易地获得总共500-1000个TMA切片。结论这种低成本和节省时间的方法为高产量TMA提供了另一种采样工具。虚拟载玻片本文的虚拟载玻片可以在这里找到： http://www.diagnosticpathology.diagnomx.eu/vs/1979605867857990
BackgroundTissue microarray (TMA) is a high throughput research tool, which has greatly facilitated and acceleratedin situtissue analyses. However, its productivity has been restricted due to the confined thickness of traditional donor block. Here, we introduce an improved high output TMA method that is applicable to a broader range of tissue samples.MethodsIn this method, a 3.6 cm long and 2.7 cm wide recipient block with 88 square lattices (3 mm in width) was first prepared using several commercial instruments. A 2 mm wide and 6 mm long tissue rod was then prepared using a self-made blade-shaped knife from each paraffin embedded donor block of gastrointestinal stromal tumors. These rods were manually arrayed one by one into the corresponding lattices of the 60°C pre-softened recipient block with the guide of holes drilled with a steel needle. A 70-rod TMA was made to testify this method.ResultsThe prepared TMA had well defined array configurations, good tissue morphology and fully preserved proteins and DNA. A total of 500–1000 TMA sections could be easily obtained from a TMA block.ConclusionThis low-cost and time-saving method provides an alternative sampling tool for high output TMA.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1979605867857990
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影响因子：
6.4
作者：
Faratian, Dana;Graham, Ashley;Thomas, Jeremy
通讯作者：
Thomas, Jeremy
DOI：
10.1007/978-1-60761-806-5_13
发表时间：
2010-01-01
期刊：
TISSUE MICROARRAYS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Kallioniemi, Anne
通讯作者：
Kallioniemi, Anne
影响因子：
82.9
作者：
Kononen, J;Bubendorf, L;Kallioniemi, OP
通讯作者：
Kallioniemi, OP
影响因子：
1.6
作者：
Jawhar NM
通讯作者：
Jawhar NM
影响因子：
2.6
作者：
Shebl AM;Zalata KR;Amin MM;El-Hawary AK
通讯作者：
El-Hawary AK