Robertsonian Fusion and Centromere Repositioning Contributed to the Formation of Satellite-free Centromeres During the Evolution of Zebras.
Robertsonian Fusion and Centromere Repositioning Contributed to the Formation of Satellite-free Centromeres During the Evolution of Zebras.
复制标题
罗伯逊融合和着丝粒重新定位有助于斑马进化过程中无卫星着丝粒的形成。
DOI：
10.1093/molbev/msac162
复制
发表时间：
2022-08-03
影响因子：
10.7
通讯作者：
Giulotto, Elena
中科院分区：
文献类型：
--
作者：
Cappelletti, Eleonora;Piras, Francesca M.;Sola, Lorenzo;Santagostino, Marco;Abdelgadir, Wasma A.;Raimondi, Elena;Lescai, Francesco;Nergadze, Solomon G.;Giulotto, Elena
着丝粒由组蛋白H3变体CENP-A表观遗传学指定，并且通常与高度重复的卫星DNA相关。我们以前发现自然无卫星neocentromeres在马caballus和马asinus。在这里，通过使用抗CENP-A抗体的ChIP-seq，我们在斑马中发现了非常高数量的缺少卫星DNA的着丝粒（22个中的15个）和斑马（23个中的13个），这表明大多数着丝粒缺少卫星DNA与基因组稳定性和物种生存相容，并挑战了卫星DNA在着丝粒功能中的作用。这两个物种之间共有9个无卫星着丝粒，与它们最近的分离一致。我们组装了E. burchelli。CENP-A结合结构域的序列分析显示它们富含LINE-1和AT，其中四个显示DNA扩增。在这两个斑马，无卫星着丝粒出现着丝粒重新定位或罗伯逊融合。在五条染色体中，着丝粒功能出现在融合点附近，融合点位于由祖先着丝粒周围序列的痕迹标记的区域内。因此，除了着丝粒重新定位，罗伯逊融合是一个重要的来源，无卫星的着丝粒在进化过程中。最后，在一种情况下，无随体的着丝粒被接种在倒位断点上。在11条染色体上，其初级缩窄似乎与卫星重复细胞遗传学分析，无卫星的新着丝粒，而不是位于附近的祖先失活的卫星为基础的着丝粒，因此，着丝粒的功能已经从一个卫星重复基因座转移到一个无卫星的新位置。
Centromeres are epigenetically specified by the histone H3 variant CENP-A and typically associated with highly repetitive satellite DNA. We previously discovered natural satellite-free neocentromeres in Equus caballus and Equus asinus. Here, through ChIP-seq with an anti-CENP-A antibody, we found an extraordinarily high number of centromeres lacking satellite DNA in the zebras Equus burchelli (15 of 22) and Equus grevyi (13 of 23), demonstrating that the absence of satellite DNA at the majority of centromeres is compatible with genome stability and species survival and challenging the role of satellite DNA in centromere function. Nine satellite-free centromeres are shared between the two species in agreement with their recent separation. We assembled all centromeric regions and improved the reference genome of E. burchelli. Sequence analysis of the CENP-A binding domains revealed that they are LINE-1 and AT-rich with four of them showing DNA amplification. In the two zebras, satellite-free centromeres emerged from centromere repositioning or following Robertsonian fusion. In five chromosomes, the centromeric function arose near the fusion points, which are located within regions marked by traces of ancestral pericentromeric sequences. Therefore, besides centromere repositioning, Robertsonian fusions are an important source of satellite-free centromeres during evolution. Finally, in one case, a satellite-free centromere was seeded on an inversion breakpoint. At 11 chromosomes, whose primary constrictions seemed to be associated with satellite repeats by cytogenetic analysis, satellite-free neocentromeres were instead located near the ancestral inactivated satellite-based centromeres; therefore, the centromeric function has shifted away from a satellite repeat containing locus to a satellite-free new position.
登录
查看更多内容
影响因子：
14.9
作者：
Barbosa AC;Xu Z;Karari K;Williams W;Hauf S;Brown WRA
通讯作者：
Brown WRA
影响因子：
14.9
作者：
CORPET, F
通讯作者：
CORPET, F
影响因子：
1.3
作者：
Cerutti F;Gamba R;Mazzagatti A;Piras FM;Cappelletti E;Belloni E;Nergadze SG;Raimondi E;Giulotto E
通讯作者：
Giulotto E
DOI：
10.1073/pnas.98.1.171
发表时间：
2001-01-02
影响因子：
11.1
作者：
Garagna, S;Marziliano, N;Redi, CA
通讯作者：
Redi, CA
DOI：
10.1016/j.cub.2014.08.017
发表时间：
2014-10-06
期刊：
Current biology : CB
影响因子：
--
作者：
Chmátal L;Gabriel SI;Mitsainas GP;Martínez-Vargas J;Ventura J;Searle JB;Schultz RM;Lampson MA
通讯作者：
Lampson MA