Hydrogels with Reversible Mechanics to Probe Dynamic Cell Microenvironments.
Hydrogels with Reversible Mechanics to Probe Dynamic Cell Microenvironments.
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DOI：
10.1002/anie.201705684
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发表时间：
2017-09-25
期刊：
影响因子：
--
通讯作者：
Anseth KS
中科院分区：
文献类型：
--
作者：
Rosales AM;Vega SL;DelRio FW;Burdick JA;Anseth KS
ECM 力学和细胞行为之间的关系是动态的，因为细胞会重塑并对其局部环境的变化做出反应。大多数体外基质是静态的且具有超生理刚性；因此，需要具有动态和可逆机械变化的平台。在这里，我们开发了基于透明质酸的基质，能够连续进行光降解和光引发交联反应，在生理相关的模量范围内“软化”然后“硬化”水凝胶。人类间充质干细胞证明了对粘附细胞的可逆机械信号传导。原位水凝胶软化（从约 14 kPa 至 3.5 kPa）导致细胞面积和 YAP/TAZ 的核定位减少，随后的硬化（从约 3.5 kPa 至 28 kPa）增加了 YAP/TAZ 的细胞面积和核定位。每个光反应都是细胞相容性和可调的，使该平台适合研究多种细胞类型和环境下细胞行为的动态力学。具有可逆力学的水凝胶是由能够光降解和光聚合的透明质酸聚合物开发而成，能够捕获天然微环境的动态方面。当基质力学发生原位变化时，人类间充质干细胞证明了细胞机械传感。
The relationship between ECM mechanics and cell behavior is dynamic, as cells remodel and respond to changes in their local environment. Most in vitro substrates are static and supraphysiologically stiff; thus, platforms with dynamic and reversible mechanical changes are needed. Here, we developed hyaluronic acid-based substrates capable of sequential photodegradation and photoinitiated crosslinking reactions to “soften” and then “stiffen” the hydrogels over a physiologically-relevant range of moduli. Reversible mechanical signaling to adhered cells was demonstrated with human mesenchymal stem cells. In situ hydrogel softening (from ~14 to 3.5 kPa) led to a decrease in cell area and nuclear localization of YAP/TAZ, and subsequent stiffening (from ~3.5 to 28 kPa) increased cell area and nuclear localization of YAP/TAZ. Each photoreaction was cytocompatible and tunable, rendering this platform amenable to studies of dynamic mechanics on cell behavior across many cell types and contexts. Hydrogels with reversible mechanics have been developed from hyaluronic acid polymers capable of photodegradation and photopolymerization, capturing dynamic aspects of native microenvironments. Cellular mechanosensing was demonstrated with human mesenchymal stem cells as the substrate mechanics changed in situ.
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影响因子：
10
作者：
Abdeen, Amr A.;Lee, Junmin;Bharadwaj, N. Ashwin;Ewoldt, Randy H.;Kilian, Kristopher A.
通讯作者：
Kilian, Kristopher A.
DOI：
10.1039/c6ib00027d
发表时间：
2016-06-13
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Caliari SR;Perepelyuk M;Soulas EM;Lee GY;Wells RG;Burdick JA
通讯作者：
Burdick JA
影响因子：
16.6
作者：
Guvendiren, Murat;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
2.8
作者：
Cloyd, Jordan M.;Malhotra, Neil R.;Elliott, Dawn M.
通讯作者：
Elliott, Dawn M.
影响因子：
15
作者：
Yoshikawa, Hiroshi Y.;Rossetti, Fernanda F.;Tanaka, Motomu
通讯作者：
Tanaka, Motomu