Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling.
Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling.
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作者：
磁小体是纳米尺寸的磁性纳米颗粒，具有精致的特性，可用于广泛的医疗保健和生物技术应用。它们由趋磁细菌（MTB）生物合成，例如磁趋菌（Magnetoetrachellum gryphiswaldense）MSR-1（Mgrenum）。然而，与磁性纳米颗粒的化学合成相比，磁小体生物加工产生的量较低。因此，了解细胞内代谢产物和代谢网络与磁小体生长和磁小体形成是至关重要的解锁这种生物体的潜力，以开发改进的生物过程。在这项工作中，我们使用非靶向代谢组学研究了镁的代谢。进行液相色谱-质谱法（LC-MS）以分析分别对应于磁小体产生细胞和非磁小体产生细胞的在O2限制（n = 6）和富O2条件（n = 6）下生长的嗜铬细胞的用过的培养基样品。进行了多变量、单变量和途径富集分析，以确定显著改变的代谢物和途径。使用真实标准品、Mgryph特定代谢物数据库和MS/MS mzCloud数据库进行严格的代谢物鉴定。PCA和OPLS-DA的交叉验证值分别为R2 X = 0.76、R2 Y = 0.99和Q2 = 0.98，表明样品组具有明显的分离性和聚类性。氨酰-tRNA生物合成和丙酮酸和柠檬酸循环（TCA）代谢。我们的研究结果表明，LC-MS的潜力，以确定关键代谢物在Mgranulum，并将有助于进一步了解影响Mgranulum的生长和磁小体形成的代谢机制。在微氧（O2限制）生长条件下，能够形成磁小体，从而显着改变代谢途径在Magnetostellum gryphiswaldense MSR-1。
Magnetosomes are nano-sized magnetic nanoparticles with exquisite properties that can be used in a wide range of healthcare and biotechnological applications. They are biosynthesised by magnetotactic bacteria (MTB), such as Magnetospirillum gryphiswaldense MSR-1 (Mgryph). However, magnetosome bioprocessing yields low quantities compared to chemical synthesis of magnetic nanoparticles. Therefore, an understanding of the intracellular metabolites and metabolic networks related to Mgryph growth and magnetosome formation are vital to unlock the potential of this organism to develop improved bioprocesses. In this work, we investigated the metabolism of Mgryph using untargeted metabolomics. Liquid chromatography-mass spectrometry (LC-MS) was performed to profile spent medium samples of Mgryph cells grown under O2-limited (n = 6) and O2-rich conditions (n = 6) corresponding to magnetosome- and non-magnetosome producing cells, respectively. Multivariate, univariate and pathway enrichment analyses were conducted to identify significantly altered metabolites and pathways. Rigorous metabolite identification was carried out using authentic standards, the Mgryph-specific metabolite database and MS/MS mzCloud database. PCA and OPLS-DA showed clear separation and clustering of sample groups with cross-validation values of R2X = 0.76, R2Y = 0.99 and Q2 = 0.98 in OPLS-DA. As a result, 50 metabolites linked to 45 metabolic pathways were found to be significantly altered in the tested conditions, including: glycine, serine and threonine; butanoate; alanine, aspartate and glutamate metabolism; aminoacyl-tRNA biosynthesis and; pyruvate and citric acid cycle (TCA) metabolisms. Our findings demonstrate the potential of LC-MS to characterise key metabolites in Mgryph and will contribute to further understanding the metabolic mechanisms that affect Mgryph growth and magnetosome formation. Metabolic pathways in Magnetospirillum gryphiswaldense MSR-1 are significantly altered under microaerobic (O2-limited) growth conditions enabling magnetosome formation.
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影响因子：
4.5
作者：
Raschdorf O;Forstner Y;Kolinko I;Uebe R;Plitzko JM;Schüler D
通讯作者：
Schüler D
DOI：
10.1007/s11306-015-0827-2
发表时间：
2015
期刊：
Metabolomics : Official journal of the Metabolomic Society
影响因子：
--
作者：
Kim DH;Achcar F;Breitling R;Burgess KE;Barrett MP
通讯作者：
Barrett MP
影响因子：
3.2
作者：
BALKWILL, DL;MARATEA, D;BLAKEMORE, RP
通讯作者：
BLAKEMORE, RP
影响因子：
4.4
作者：
Lohsse, Anna;Kolinko, Isabel;Schueler, Dirk
通讯作者：
Schueler, Dirk
影响因子：
4.1
作者：
Kessler, B;Witholt, B
通讯作者：
Witholt, B