Ex vivo expansion of cord blood.
Ex vivo expansion of cord blood.
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DOI：
10.1038/bmt.2009.284
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发表时间：
2009-11
影响因子：
4.8
通讯作者：
Shpall E
中科院分区：
文献类型：
--
作者：
Kelly SS;Sola CB;de Lima M;Shpall E
近年来，人们观察到脐带血（UCB）移植的利用率显著增加;然而，使用UCB作为造血干细胞（HSC）来源主要受到移植物中所含祖细胞数量的限制。移植失败、延迟植入和免疫重建的严重延迟导致成人的显著发病率和死亡率。缺乏可用于移植后治疗的细胞，如供体淋巴细胞输注，也被认为是UCB的缺点。为了改善UCB移植的结果并扩展其适用性，一个潜在的解决方案是UCB的离体扩增。研究人员已经使用了几种方法，包括液体悬浮培养与各种细胞因子和扩增因子，共培养与基质元素和连续灌注系统。正在探索将离体扩增和未操作的UCB相结合的技术，以优化初始植入动力学以及长期耐久性。最佳扩增条件仍然未知;然而，最近的研究表明，扩增的UCB是安全的。希望通过离体扩增UCB，将观察到UCB移植的发病率和死亡率的降低，并且额外细胞的可用性可能允许在UCB环境中进行过继免疫治疗或基因转移治疗。
A marked increase in the utilization of umbilical cord blood (UCB) transplantation has been observed in recent years; however, the use of UCB as a hematopoietic stem cell (HSC) source is limited primarily by the number of progenitor cells contained in the graft. Graft failure, delayed engraftment and profound delay in immune reconstitution lead to significant morbidity and mortality in adults. The lack of cells available for post transplant therapies, such as donor lymphocyte infusions, has also been considered to be a disadvantage of UCB. To improve outcomes and extend applicability of UCB transplantation, one potential solution is ex vivo expansion of UCB. Investigators have used several methods, including liquid suspension culture with various cytokines and expansion factors, co-culture with stromal elements and continuous perfusion systems. Techniques combining ex vivo expanded and unmanipulated UCB are being explored to optimize the initial engraftment kinetics as well as the long-term durability. The optimal expansion conditions are still not known; however, recent studies suggest that expanded UCB is safe. It is hoped that by ex vivo expansion of UCB, a resulting decrease in the morbidity and mortality of UCB transplantation will be observed, and that the availability of additional cells may allow adoptive immunotherapy or gene transfer therapies in the UCB setting.
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影响因子：
1.5
作者：
Cohen, Yossi;Kreiser, Doron;Nagler, Arnon
通讯作者：
Nagler, Arnon
影响因子：
4.8
作者：
de Lima, M.;McMannis, J.;Shpall, E. J.
通讯作者：
Shpall, E. J.
影响因子：
5.6
作者：
DEXTER, TM;ALLEN, TD;LORD, BI
通讯作者：
LORD, BI
DOI：
10.1073/pnas.86.10.3828
发表时间：
1989-05-01
影响因子：
11.1
作者：
BROXMEYER, HE;DOUGLAS, GW;BOYSE, EA
通讯作者：
BOYSE, EA
DOI：
10.1002/stem.5530080708
发表时间：
1990-01-01
期刊：
INTERNATIONAL JOURNAL OF CELL CLONING
影响因子：
--
作者：
BROXMEYER, HE;GLUCKMAN, E;BOYSE, EA
通讯作者：
BOYSE, EA