Intracellular glucose starvation affects gingival homeostasis and autophagy.
Intracellular glucose starvation affects gingival homeostasis and autophagy.
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DOI：
10.1038/s41598-022-05398-2
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发表时间：
2022-01-24
影响因子：
4.6
通讯作者：
Umeda M
中科院分区：
文献类型：
--
作者：
Li R;Kato H;Taguchi Y;Umeda M
人牙龈成纤维细胞（HGnFs）通过活跃的增殖和迁移维持牙周组织的稳态。临床上认为，即使在牙周手术后不久营养物（例如葡萄糖）供应不足的环境中，牙龈组织的伤口愈合能力也得以维持。然而，这种葡萄糖缺乏环境对HGnFs的影响仍不清楚。本研究旨在探讨低糖环境对促性腺激素释放因子稳态的影响。我们评估牙龈伤口愈合检查细胞增殖和迁移和胶原蛋白的合成，在100，50，25，和0毫克/分升的葡萄糖在体外培养的HGnFs。通过测定乳酸脱氢酶（LDH）和活性氧（ROS）水平来确定细胞应激水平。通过测定葡萄糖转运蛋白1（GLUT 1）mRNA表达、葡萄糖摄取试验、乳酸和ATP产生来研究低葡萄糖浓度下HGnFs的葡萄糖代谢。研究了分子效应，重点是LKB 1-AMPK信号通路。通过测量自噬相关蛋白的水平来评估葡萄糖剥夺的HGnFs中的自噬活性。低葡萄糖水平增加细胞应激水平、自噬活性，并通过LKB 1-AMPK信号通路增强葡萄糖代谢，提供更多ATP以促进伤口愈合。我们关于葡萄糖转移的研究结果表明牙龈伤口的快速愈合。
Human gingival fibroblasts (HGnFs) maintain periodontal tissue homeostasis through active proliferation and migration. Clinically, it is considered that the wound-healing ability of the gingival tissue is maintained even in environments with insufficient supply of nutrients, such as glucose, immediately after periodontal surgery. However, the effects of such glucose-deficient environments on HGnFs remain unclear. This study aimed to investigate the effects of low-glucose environment on HGnFs homeostasis. We evaluated gingival wound healing by examining cell proliferation and migration and collagen synthesis in HGnFs cultured in 100, 50, 25, and 0 mg/dL glucose in vitro. The cellular stress levels were determined by measuring the lactate dehydrogenase (LDH) and reactive oxygen species (ROS) levels. The glucose metabolism of HGnFs in the low-glucose concentrations was studied by measuring glucose transporter type 1 (GLUT1) mRNA expression, glucose uptake assays, lactate and ATP productions. Molecular effects were examined with a focus on the LKB1-AMPK signaling pathway. Autophagy activity in glucose-deprived HGnFs was evaluated by measuring the levels of autophagy-related proteins. Low glucose levels increased cellular stress levels, autophagy activity, and enhanced glucose metabolism through the LKB1-AMPK signaling pathway, providing more ATPs to promote wound healing. Our results regarding glucose transfer suggest the rapid healing of gingival wounds.
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DOI：
10.1083/jcb.152.4.657
发表时间：
2001-02-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mizushima N;Yamamoto A;Hatano M;Kobayashi Y;Kabeya Y;Suzuki K;Tokuhisa T;Ohsumi Y;Yoshimori T
通讯作者：
Yoshimori T
影响因子：
4.6
作者：
Endo H;Owada S;Inagaki Y;Shida Y;Tatemichi M
通讯作者：
Tatemichi M
影响因子：
78.5
作者：
Shackelford, David B.;Shaw, Reuben J.
通讯作者：
Shaw, Reuben J.
影响因子：
20.1
作者：
NEELY, JR;GROTYOHANN, LW
通讯作者：
GROTYOHANN, LW
AMPK：代谢和线粒体稳态的监护人。
DOI：
10.1038/nrm.2017.95
发表时间：
2018-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Herzig S;Shaw RJ
通讯作者：
Shaw RJ