Bacterial/archaeal/organellar polyadenylation.
Bacterial/archaeal/organellar polyadenylation.
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DOI：
10.1002/wrna.51
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发表时间：
2011-03
影响因子：
7.3
通讯作者：
Kushner, Sidney R.
中科院分区：
文献类型：
--
作者：
Mohanty, Bijoy K.;Kushner, Sidney R.
尽管 1962 年在大肠杆菌中发现了第一个聚腺苷酸聚合酶 (PAP)，但在接下来的 30 年里，细菌中聚腺苷酸化的研究基本上被忽视了。然而，随着 1992 年大肠杆菌 PAP I 结构基因的鉴定，使用生化和遗传学方法分析聚腺苷酸化成为可能。随后，研究表明多腺苷酸化在原核RNA代谢中发挥多功能作用。虽然我们目前对原核多聚腺苷酸化的理解大部分来自于对大肠杆菌的研究，但最近对蓝细菌、细胞器和古细菌的实验虽然有限，但拓宽了我们对多聚腺苷酸化过程的多样性、复杂性和普遍性的看法。例如，多核苷酸磷酸化酶（PNPase）（一种在细菌中高度保守的可逆磷酸化酶）被鉴定为大肠杆菌中的额外 PAP，令所有人感到惊讶。事实上，PNPase 现在已被证明是多种原核细胞（包括那些缺乏真细菌 PAP 同源物的细胞）中转录后 RNA 修饰的来源。因此，在过去的几年里，人们对所有原核物种中转录后修饰的机制和作用越来越感兴趣。然而，许多多聚腺苷酸尾非常短且不稳定，以及多核苷酸尾的存在，这一事实给试图解开原核生物多聚腺苷酸化之谜的科学界带来了重大的技术挑战。这篇综述讨论了有关聚腺苷酸化及其在细菌、细胞器和古细菌中的功能的当前知识状况。
Although the first poly(A) polymerase (PAP) was discovered in Escherichia coli in 1962, the study of polyadenylation in bacteria was largely ignored for the next 30 years. However, with the identification of the structural gene for E. coli PAP I in 1992, it became possible to analyze polyadenylation using both biochemical and genetic approaches. Subsequently, it has been shown that polyadenylation plays a multifunctional role in prokaryotic RNA metabolism. While the bulk of our current understanding of prokaryotic polyadenylation comes from studies on E. coli, recent experiments with Cyanobacteria, organelles and Archaea, although limited, have widened our view on the diversity, complexity, and universality of the polyadenylation process. For example, the identification of polynucleotide phosphorylase (PNPase), a reversible phosphorolytic enzyme that is highly conserved in bacteria, as an additional PAP in E. coli caught everyone by surprise. In fact, PNPase has now been shown to be the source of post-transcriptional RNA modifications in a wide range of cells of prokaryotic origin including those that lack a eubacterial PAP homologue. Accordingly, the past few years have witnessed increased interest in the mechanism and role of post-transcriptional modifications in all species of prokaryotic origin. However, the fact that many of the poly(A) tails are very short and unstable as well as the presence of polynucleotide tails has posed significant technical challenges to the scientific community trying to unravel the mystery of polyadenylation in prokaryotes. This review discusses the current state of knowledge regarding polyadenylation and its functions in bacteria, organelles and Archaea.
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影响因子：
16
作者：
Betat, H;Rammelt, C;Mörl, M
通讯作者：
Mörl, M
DOI：
10.1073/pnas.93.21.11580
发表时间：
1996-10-15
影响因子：
11.1
作者：
Cao, GJ;Pogliano, J;Sarkar, N
通讯作者：
Sarkar, N
影响因子：
3.2
作者：
Carabetta, Valerie J.;Mohanty, Bijoy K.;Silhavy, Thomas J.
通讯作者：
Silhavy, Thomas J.
影响因子：
19
作者：
Chanfreau, GF
通讯作者：
Chanfreau, GF
影响因子：
4.8
作者：
Blum, E;Carpousis, AJ;Higgins, CF
通讯作者：
Higgins, CF