Cellular toxicity and lipid peroxidation in response to mercury.
Cellular toxicity and lipid peroxidation in response to mercury.
复制标题
汞引起的细胞毒性和脂质过氧化。
DOI：
10.1016/0041-008x(82)90023-0
复制
发表时间：
1982
影响因子：
3.8
通讯作者：
Hermann Kappus
中科院分区：
文献类型：
--
作者：
N. Stacey;Hermann Kappus
It has been proposed that the peroxidation of lipids may account for the toxicity of the heavy metal, mercury. Recent evidence has discounted a similar theory for another heavy metal, cadmium, when examined using isolated rat hepatocytes. Such a system provides the opportunity to examine several parameters and treatments simultaneously on aliquots from the same parent population of hepatocytes. Thus, isolated hepatocytes were incubated with mercuric chloride at concentrations ranging from 10 to 200 μm for 20, 40, or 60 min of incubation. Lipid peroxidation was found to increase with the concentration of HgCl2and incubation time, as assessed by increases in thiobarbituric acid-reacting substances and in ethane in the gas phase of the flasks. Cell viability was reduced by Hg2+as determined by the release of cytoplasmic lactate dehydrogenase from hepatocytes. Reduced glutathione also declined in the presence of the higher concentrations of Hg2+, suggesting this decrease to be a manifestation of the toxic response. The increases in thiobarbituric acid reactant concentrations were closely related to loss of cytoplasmic lactate dehydrogenase, suggesting a possible causative role for lipid peroxidation in the toxic response. However, addition of the antioxidant N,N′-diphenyl-p-phenylenediamine or (+)-cyanidanol-3 to the cell suspensions inhibited lipid peroxidation, but only (+)-cyanidanol-3 could partially reduce the Hg2+-induced cytotoxicity. This finding indicates that the lipid peroxidation associated with loss of viability of isolated rat hepatocytes is not directly responsible for this cell injury.
抑制脂质过氧化而不预防离体大鼠肝细胞的细胞损伤。
影响因子：
3.8
作者：
Stacey,NH;Klaassen,CD
通讯作者：
Klaassen,CD
比较金属对离体大鼠肝细胞细胞损伤和脂质过氧化的影响。
DOI：
10.1080/15287398109529965
发表时间：
1981
期刊：
Journal of toxicology and environmental health
影响因子：
--
作者：
Stacey,NH;Klaassen,CD
通讯作者：
Klaassen,CD