Tumor-Infiltrating Lymphocytes Express Vascular Endothelial Growth Factor in Renal Cell Carcinomas
Tumor-Infiltrating Lymphocytes Express Vascular Endothelial Growth Factor in Renal Cell Carcinomas
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肾细胞癌中肿瘤浸润淋巴细胞表达血管内皮生长因子
DOI：
10.1159/000027212
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发表时间：
2000
影响因子：
2.4
通讯作者：
T. Otto
中科院分区：
文献类型：
--
作者：
G. Lümmen;S. Blass;H. Rübben;J. Suhr;T. Otto
背景资料：免疫系统对肾细胞癌（RCC）转移的已知影响已经导致了几种治疗方法的发展，用于通过细胞因子和肿瘤浸润淋巴细胞（TIL）的回输进一步刺激宿主免疫系统。基于对人前列腺癌和移行细胞癌的观察，我们研究了不同阶段RCC中TIL的存在与血管内皮生长因子（VEGF）表达的相关性，作为RCC患者肿瘤进展和不良生存的参数。研究方法：将手术获得的RCC（n = 28）和良性肾实质（n =10）的样本快速冷冻，并通过CD 4/CD 8和VEGF抗体的双重免疫荧光染色进行处理。结果：28例肾癌中有20例同时表达TIL特异性标志物CD 4和/或CD 8和VEGF。对照组织为VEGF阴性，仅显示可忽略的CD 4或CD 8阳性淋巴细胞浸润。结论：结果表明，至少71%的TIL产生VEGF，并可能促进肿瘤进展，而不是代表宿主免疫系统的流产抗肿瘤反应。
Background: Known influence of the immune system on metastases of renal cell cancer (RCC) has led to the development of several therapeutic approaches for further stimulation of the host immune system by cytokines and the retransfusion of tumor-infiltrating lymphocytes (TIL). Based on observations in human prostate adenocarcinomas and transitional cell carcinomas, we have investigated the presence of TIL in different stages of RCC in correlation to vascular endothelial growth factor (VEGF) expression as a parameter for tumor progression and adverse survival in RCC patients. Methods: Samples from surgically obtained RCC (n = 28) and benign renal parenchyma (n =10) were snap-frozen and processed by double-immunofluorescence staining with CD4/CD8 and VEGF antibodies. Results: In 20 of 28 RCCs a coexpression of TIL-specific markers CD4 and/or CD8 and VEGF was demonstrated. Control tissues were VEGF-negative and showed only negligible infiltration by CD4- or CD8-positive lymphocytes. Conclusion: The results indicate that at least 71% of TIL produce VEGF and may promote tumor progression rather than represent an abortive antitumor response of the host immune system.
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影响因子：
6.6
作者：
Figlin, RA;Pierce, WC;Belldegrun, A
通讯作者：
Belldegrun, A
影响因子：
4.4
作者：
G. Peoples;P. Goedegebuure;J. Andrews;D. Schoof;T. Eberlein
通讯作者：
G. Peoples;P. Goedegebuure;J. Andrews;D. Schoof;T. Eberlein
DOI：
10.1073/pnas.91.8.2890
发表时间：
1994-04-12
影响因子：
11.1
作者：
BLOTNICK, S;PEOPLES, GE;KLAGSBRUN, M
通讯作者：
KLAGSBRUN, M
浸润肿瘤和动脉粥样硬化斑块的 T 淋巴细胞产生肝素结合表皮生长因子样生长因子和碱性成纤维细胞生长因子：潜在的病理作用。
DOI：
10.1073/pnas.92.14.6547
发表时间：
1995
影响因子：
11.1
作者：
Peoples,GE;Blotnick,S;Takahashi,K;Freeman,MR;Klagsbrun,M;Eberlein,TJ
通讯作者：
Eberlein,TJ