Characterization of a novel ultra low refractive index material for biosensor application.
Characterization of a novel ultra low refractive index material for biosensor application.
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DOI：
10.1016/j.snb.2009.04.034
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发表时间：
2009-08-18
影响因子：
8.4
通讯作者：
Grant, Sheila A.
中科院分区：
文献类型：
--
作者：
Memisevic, Jasenka;Korampally, Venumadhav;Gangopadhyay, Shubhra;Grant, Sheila A.
纳米多孔材料可以为生物传感器领域提供显著的益处。它们的尺寸和多孔结构使其成为改善传感器性能的理想工具。本研究的特点是一种新型的超低折射率的纳米多孔有机硅（NPO）材料，用作基于荧光的光学生物传感器的光学平台。在用作低折射率包层材料的同时，基于有机硅酸盐纳米颗粒的新型涂层还为可用于固定生物探针的高表面积涂层提供了机会。将生物分子固定在NPO上，NPO旋涂在硅和玻璃基底上。生物分子由蛋白A与AlexaFluor 546荧光团缀合组成，然后通过硅烷化固定到NPO底物上。样品分析包括荧光分光光度法、FT-IR光谱法、扫描电子显微镜、接触角测量和椭圆偏振法。结果显示在574 nm处存在发射峰，表明蛋白A固定到NPO材料上是可能的。当与未涂覆有NPO的Si和玻璃基板相比时，结果显示，涂覆有NPO的膜的堆积密度分别增加了100倍和10倍。表面固定的NPO膜的椭圆偏振分析、FT-IR、接触角和SEM成像表明，虽然表面改性确实引起了一些损害，但其独特特性没有发生显著变化，即，孔隙结构、润湿性和折射率。结果表明，NPO薄膜是一种可行的传感器衬底，以提高灵敏度和改善传感器的性能。
Nanoporous materials can provide significant benefits to the field of biosensors. Their size and porous structure makes them an ideal tool for improving sensor performance. This study characterized a novel ultra low index of refraction nanoporous organosilicate (NPO) material for use as an optical platform for fluorescence-based optical biosensors. While serving as the low index cladding material, the novel coating based on organosilicate nanoparticles also provides an opportunity for a high surface area coating that can be utilized for immobilizing biological probes. Biological molecules were immobilized onto NPO, which was spin-coated on silicon and glass substrates. The biological molecule was composed of Protein A conjugated to AlexaFluor 546 fluorophore and then immobilized onto the NPO substrate via silanization. Sample analysis consisted of spectrofluorometry, FT-IR spectroscopy, scanning electron microscopy, contact angle measurement and ellipsometry. The results showed the presence of emission peaks at 574 nm, indicating that the immobilization of Protein A to the NPO material is possible. When compared to Si and glass substrates not coated with NPO, the results showed a 100X and 10X increase in packing density with the NPO coated films respectively. Ellipsometric analysis, FT-IR, contact angle, and SEM imaging of the surface immobilized NPO films suggested that while the surface modifications did induce some damage, it did not incur significant changes to its unique characteristics, i.e., pore structure, wettability and index of refraction. It was concluded that NPO films would be a viable sensor substrate to enhance sensitivity and improve sensor performance.
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影响因子：
6.2
作者：
Larsson, H;Dasgupta, PK
通讯作者：
Dasgupta, PK
影响因子：
3.8
作者：
Risk, WP;Kim, HC;Gangopadhyay, S
通讯作者：
Gangopadhyay, S
影响因子：
7.4
作者：
Liu, Z;Pawliszyn, J
通讯作者：
Pawliszyn, J
影响因子：
2.9
作者：
BHATIA, SK;SHRIVERLAKE, LC;LIGLER, FS
通讯作者：
LIGLER, FS
影响因子：
12.6
作者：
Di Francia, G;La Ferrara, V;Chiavarini, S
通讯作者：
Chiavarini, S