Expression of leptin receptor gene in developing and adult zebrafish.
Expression of leptin receptor gene in developing and adult zebrafish.
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DOI：
10.1016/j.ygcen.2009.11.015
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发表时间：
2010-04-01
影响因子：
2.7
通讯作者：
Londraville, Richard L.
中科院分区：
文献类型：
--
作者：
Liu, Qin;Chen, Yun;Copeland, Donald;Ball, Hope;Duff, Robert J.;Rockich, Briana;Londraville, Richard L.
瘦素和瘦素受体的相互作用在动物发育和调节食欲和能量平衡中起着至关重要的作用。本研究采用原位杂交和Q-PCR方法分析了斑马鱼瘦素受体基因在发育和成年斑马鱼中的表达模式。斑马鱼瘦素受体信息（lepr）在所有胚胎和幼虫阶段以及成年斑马鱼中都被检测到。在胚胎斑马鱼中，麻风基因主要在脊索中表达。随着发育的进行，麻风基因在脊索中的表达减少，而在其他组织中的表达，包括躯干肌肉和肠道，变得明显。在幼虫和成年的大脑中，在后脑的类似区域检测到大的表达麻风细胞。在成年斑马鱼中，在包括下丘脑外侧结节核（弓形核的鱼类同系物）在内的其他几个大脑区域也观察到麻风基因的表达。Q-PCR实验证实了麻风基因在成年鱼的大脑中表达，并且在包括肝脏、肌肉和性腺在内的几个成年组织中也有表达。结果表明，麻风基因的表达受时间和空间调控。
Interactions of leptin and leptin receptors play crucial roles during animal development and regulation of appetite and energy balance. In this study we analyzed expression pattern of a zebrafish leptin receptor gene in both developing and adult zebrafish using in situ hybridization and Q-PCR methods. Zebrafish leptin receptor message (lepr) was detected in all embryonic and larval stages examined, and in adult zebrafish. In embryonic zebrafish, lepr was mainly expressed in the notochord. As development proceeded, lepr expression in the notochord decreased, while its expression in several other tissues, including the trunk muscles and gut, became evident. In both larval and adult brains, large lepr expressing cells were detected in similar regions of the hindbrain. In adult zebrafish, lepr expression was also observed in several other brain regions including the hypothalamic lateral tuberal nucleus, the fish homolog of the arcuate nucleus. Q-PCR experiments confirmed lepr expression in the adult fish brain, and also showed lepr expression in several adult tissues including liver, muscle and gonads. Our results showed that lepr expression was both spatially and temporally regulated.
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影响因子：
3.2
作者：
Hansen, Gert H.;Niels-Christiansen, Lise-Lotte;Danielsen, E. Michael
通讯作者：
Danielsen, E. Michael
影响因子：
5.8
作者：
Aparicio, T;Kermorgant, S;Lehy, T
通讯作者：
Lehy, T
DOI：
10.1073/pnas.0507519103
发表时间：
2006-06-27
影响因子：
11.1
作者：
Crespi, Erica J.;Denver, Robert J.
通讯作者：
Denver, Robert J.
影响因子：
4.8
作者：
Grill, HJ;Schwartz, MW;Baskin, DG
通讯作者：
Baskin, DG
影响因子：
4.6
作者：
Glickman, NS;Kimmel, CB;Adams, RJ
通讯作者：
Adams, RJ