Genome-Wide Identification of Membrane-Bound Fatty Acid Desaturase Genes in Three Peanut Species and Their Expression in Arachis hypogaea during Drought Stress.
Genome-Wide Identification of Membrane-Bound Fatty Acid Desaturase Genes in Three Peanut Species and Their Expression in Arachis hypogaea during Drought Stress.
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DOI：
10.3390/genes13101718
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发表时间：
2022-09-25
期刊：
影响因子：
3.5
通讯作者：
Yuan, Yanchao
中科院分区：
文献类型：
--
作者：
Gai, Wenyu;Sun, Hua;Hu, Ya;Liu, Chunying;Zhang, Yuxi;Gai, Shupeng;Yuan, Yanchao
作为一种主要依靠雨水灌溉的作物，花生的质量和产量受到干旱的严重限制。迄今为止，许多研究表明，脂肪酸去饱和酶（FAD）基因增强植物对干旱胁迫的耐受性。在这项研究中，16个，15个，和31个FAD的确定，分别在Arachis duranensis，Arachis ipaensis，和Arachis hypogaea。所有FADs可分为4个亚家族，它们具有相对保守的基因结构、基序和结构域。同线性关系和染色体位置分析表明，A. duranensis-A. hypogaea（AA）和A. Ipaensis-A. hypogaea（BB）同源，它们的物理位置是一致的。Ka/Ks结果表明，9个FAD基因经历了纯化选择，Ah| FAD3.2在四倍体花生形成过程中经历了正选择。启动子中与激素信号和应激反应相关的各种顺式作用元件以及预测的靶向Ah的miRNA| FAD表明这些基因在耐旱性中起着关键作用。Ah的表达谱|干旱胁迫下，22个组织和耐旱、不耐旱品种的FADs分析表明，分别有4个和6个FADs是与油分积累和干旱相关的基因。这些发现将有助于深入了解FAD基因的潜在功能作用，这可能有助于应对植物干旱胁迫。
As a crop irrigated primarily by rain, the quality and yield of peanuts are significantly limited by drought. To date, many studies have indicated that fatty acid desaturase (FAD) genes enhance plant tolerance to drought stresses. In this study, 16, 15, and 31 FADs were identified in Arachis duranensis, Arachis ipaensis, and Arachis hypogaea, respectively. All the FADs were divided into four subfamilies, which had relatively conserved gene structures, motifs, and domains. The synteny relationships and chromosomal position analysis showed that the FADs in subgenome pairs, A. duranensis-A. hypogaea (AA) and A. ipaensis-A. hypogaea (BB), were homologous, and their physical locations were consistent. The Ka/Ks results indicated that nine FAD genes underwent a purifying selection, and Ah|FAD3.2 experienced positive selection during tetraploid peanut speciation. Various cis-acting elements related to hormone signaling and stress responsiveness in promoters and the predicted miRNA targeting Ah|FADs suggested that these genes play crucial roles in drought tolerance. The expression profiles of Ah|FADs in 22 tissues and drought-tolerant and -sensitive cultivars under drought stress suggested that 4 and 6 FADs were putative genes related to oil accumulation and drought, respectively. These findings will help provide insight into the potential functional roles of the FAD genes, which may aid in dealing with plant drought stress.
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