Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry.
Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry.
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DOI：
10.1371/journal.pone.0119668
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Gerwick L
中科院分区：
文献类型：
--
作者：
Mascuch SJ;Moree WJ;Hsu CC;Turner GG;Cheng TL;Blehert DS;Kilpatrick AM;Frick WF;Meehan MJ;Dorrestein PC;Gerwick L
白鼻综合征（WNS）引起的病原真菌Pseudogymnococcus destructans正在大量减少几个冬眠的北美蝙蝠物种的种群。关于病原体和宿主在该病中的分子相互作用知之甚少。荧光显微镜环境电离质谱法被用来生成代谢谱从健康和患病蝙蝠属鼠耳蝠的翅膀。在患有WNS的蝙蝠的翅膀上检测到真菌铁载体，即从环境中吸收铁的分子，但在健康蝙蝠上没有检测到。这项工作是直接从受感染宿主中检测微生物分子的第一个例子，并突出了大气电离方法提供直接分子洞察感染的能力。
White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseased bats of the genus Myotis. Fungal siderophores, molecules that scavenge iron from the environment, were detected on the wings of bats with WNS, but not on healthy bats. This work is among the first examples in which microbial molecules are directly detected from an infected host and highlights the ability of atmospheric ionization methodologies to provide direct molecular insight into infection.
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影响因子：
56.9
作者：
Harvell, CD;Kim, K;Vasta, GR
通讯作者：
Vasta, GR
影响因子：
4.9
作者：
Fluckinger, M;Haas, H;Redl, B
通讯作者：
Redl, B
影响因子：
3.1
作者：
Hissen, AHT;Wan, ANC;Moore, MM
通讯作者：
Moore, MM
DOI：
10.1038/nrmicro2836
发表时间：
2012-07-16
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
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影响因子：
56.9
作者：
Blehert, David S.;Hicks, Alan C.;Stone, Ward B.
通讯作者：
Stone, Ward B.