Set4 regulates stress response genes and coordinates histone deacetylases within yeast subtelomeres.
Set4 regulates stress response genes and coordinates histone deacetylases within yeast subtelomeres.
复制标题
SET4调节应力反应基因，并在酵母亚端子中协调组蛋白脱乙酰基酶。
DOI：
10.26508/lsa.202101126
复制
发表时间：
2021-12
影响因子：
4.4
通讯作者：
Green EM
中科院分区：
文献类型：
--
作者：
Jethmalani Y;Tran K;Negesse MY;Sun W;Ramos M;Jaiswal D;Jezek M;Amos S;Garcia EJ;Park D;Green EM
酵母染色质蛋白Set4在正常和应激条件下调节亚端粒区域内的应激反应基因的表达，并调节染色质处的组蛋白脱乙酰酶活性。酵母染色质蛋白Set4是SET结构域蛋白Set3亚家族的成员，其在不同发育和环境背景下的基因表达调控中起关键作用。我们以前报道，Set4促进生存在氧化应激和调节应激反应基因的表达，通过压力依赖的染色质定位。在这项研究中，全局基因表达分析和组蛋白修饰状态的调查确定了Set4在正常和应激条件下维持酵母亚端粒内基因抑制机制的作用。我们发现，Set4的作品在一个部分重叠的途径SIR复合物和组蛋白去乙酰化酶Rpd3保持适当水平的组蛋白乙酰化和表达的压力反应基因编码的亚端粒。Set4的这种作用对于缺氧条件下的细胞特别关键，其中Set4的损失降低了细胞适应性和细胞壁完整性。这些发现揭示了一种新的亚端粒染色质调节剂，这是应激防御途径的关键，并证明了Set4在调节抑制性异染色质样环境中的功能。
The yeast chromatin protein Set4 regulates expression of stress response genes within subtelomeric regions under normal and stress conditions and modulates histone deacetylase activity at chromatin. The yeast chromatin protein Set4 is a member of the Set3-subfamily of SET domain proteins which play critical roles in the regulation of gene expression in diverse developmental and environmental contexts. We previously reported that Set4 promotes survival during oxidative stress and regulates expression of stress response genes via stress-dependent chromatin localization. In this study, global gene expression analysis and investigation of histone modification status identified a role for Set4 in maintaining gene repressive mechanisms within yeast subtelomeres under both normal and stress conditions. We show that Set4 works in a partially overlapping pathway to the SIR complex and the histone deacetylase Rpd3 to maintain proper levels of histone acetylation and expression of stress response genes encoded in subtelomeres. This role for Set4 is particularly critical for cells under hypoxic conditions, where the loss of Set4 decreases cell fitness and cell wall integrity. These findings uncover a new regulator of subtelomeric chromatin that is key to stress defense pathways and demonstrate a function for Set4 in regulating repressive, heterochromatin-like environments.
登录
查看更多内容
影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
DOI：
10.1073/pnas.0909169107
发表时间：
2010-03-23
影响因子：
11.1
作者：
Ehrentraut, Stefan;Weber, Jan M.;Ehrenhofer-Murray, Ann E.
通讯作者：
Ehrenhofer-Murray, Ann E.
影响因子：
5.6
作者：
Jaiswal D;Turniansky R;Green EM
通讯作者：
Green EM
影响因子：
25
作者：
Deliu, Elena;Arecco, Niccolo;Novarino, Gaia
通讯作者：
Novarino, Gaia
影响因子：
16
作者：
Altaf, Mohammed;Utley, Rhea T.;Cote, Jacques
通讯作者：
Cote, Jacques