Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions.
Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions.
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DOI：
10.1186/s13578-022-00786-7
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发表时间：
2022-05-02
影响因子：
7.5
通讯作者：
Kasper, Cornelia
中科院分区：
文献类型：
--
作者：
Almeria, Ciarra;Kress, Sebastian;Weber, Viktoria;Egger, Dominik;Kasper, Cornelia
关键词：
细胞外囊泡（EVs）是细胞衍生的膜结构，作为生物分子传递到靶细胞的载体，在生理和病理过程中发挥重要作用。越来越多的先前归因于细胞疗法的效应已经被认为实际上是由来自各自细胞的ev介导的，这表明纯化的ev而不是活细胞用于细胞疗法。在这篇综述中，我们重点关注来自间充质干细胞/基质细胞（MSC）的ev的异质性，并总结了对其治疗特性和生物学功能产生关键影响的上游工艺参数。在此，我们讨论了细胞来源、培养基组成、三维培养、生物反应器培养和缺氧的影响。此外，还描述了影响电动汽车的隔离和存储策略的各个方面。最后，上游工艺参数的优化应侧重于控制msc衍生的EV异质性，以用于特定的治疗应用。
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects previously attributed to cell-based therapies have been recognized to be actually mediated by EVs derived from the respective cells, suggesting the administration of purified EVs instead of living cells for cell-based therapies. In this review, we focus on the heterogeneity of EVs derived from mesenchymal stem/stromal cells (MSC) and summarize upstream process parameters that crucially affect the resulting therapeutic properties and biological functions. Hereby, we discuss the effects of the cell source, medium composition, 3D culture, bioreactor culture and hypoxia. Furthermore, aspects of the isolation and storage strategies influences EVs are described. Conclusively, optimization of upstream process parameters should focus on controlling MSC-derived EV heterogeneity for specific therapeutic applications.
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DOI：
10.1038/mt.2008.1
发表时间：
2008-04
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
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通讯作者：
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影响因子：
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影响因子：
3.7
作者：
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DOI：
10.33594/000000102
发表时间：
2019-01-01
期刊：
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
影响因子：
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作者：
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通讯作者：
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影响因子：
13.6
作者：
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通讯作者：
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