Three-dimensional cell culture: a breakthrough in vivo.
Three-dimensional cell culture: a breakthrough in vivo.
复制标题
三维细胞培养：体内突破。
DOI：
10.3390/ijms16035517
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发表时间：
2015-03-11
影响因子：
5.6
通讯作者：
Noel G
中科院分区：
文献类型：
--
作者：
Antoni D;Burckel H;Josset E;Noel G
细胞培养是生物学研究的重要工具。二维细胞培养已经使用了一段时间，但是在塑料表面上以平坦层生长细胞并不能准确地模拟体内状态。与二维情况相比，三维（3D）细胞培养允许生物细胞生长或由于人工环境而在所有三维中与其周围环境相互作用。在3D模型中生长的细胞已被证明更具生理相关性，并在几项生物机制研究中显示出改善，如：细胞数量监测、生存力、形态学、增殖、分化、对刺激的反应、肿瘤细胞迁移和侵入周围组织、血管生成刺激和免疫系统逃避、药物代谢、基因表达和蛋白质合成，一般细胞功能和体内相关性。3D培养模型的成功得益于技术进步，包括材料科学、细胞生物学和生物反应器设计。
Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design.
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