Neuroprotective effect of rapamycin on spinal cord injury via activation of the Wnt/β-catenin signaling pathway.
Neuroprotective effect of rapamycin on spinal cord injury via activation of the Wnt/β-catenin signaling pathway.
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DOI：
10.4103/1673-5374.158360
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发表时间：
2015-06
影响因子：
6.1
通讯作者：
Mei XF
中科院分区：
文献类型：
--
作者：
Gao K;Wang YS;Yuan YJ;Wan ZH;Yao TC;Li HH;Tang PF;Mei XF
Wnt/β-catenin信号通路在神经发育、轴突导向、神经病理性疼痛缓解和神经元存活中起着至关重要的作用。在这项研究中，我们首先检查了雷帕霉素对脊髓损伤后Wnt/β-catenin信号通路的影响，通过腹膜内注射雷帕霉素脊髓损伤大鼠超过2天。Western blot和免疫荧光染色检测Wnt/β-catenin信号通路中β-catenin蛋白、caspase-3蛋白和脑源性神经营养因子蛋白的表达水平。雷帕霉素可提高损伤脊髓中β-catenin和脑源性神经营养因子的水平，改善损伤部位的病理形态，减少运动神经元的丢失，促进大鼠脊髓损伤后运动功能的恢复。我们的实验结果表明，雷帕霉素干预的神经保护作用是通过激活Wnt/β-catenin信号通路介导的脊髓损伤后。
The Wnt/β-catenin signaling pathway plays a crucial role in neural development, axonal guidance, neuropathic pain remission and neuronal survival. In this study, we initially examined the effect of rapamycin on the Wnt/β-catenin signaling pathway after spinal cord injury, by intraperitoneally injecting spinal cord injured rats with rapamycin over 2 days. Western blot analysis and immunofluorescence staining were used to detect the expression levels of β-catenin protein, caspase-3 protein and brain-derived neurotrophic factor protein, components of the Wnt/β-catenin signaling pathway. Rapamycin increased the levels of β-catenin and brain-derived neurotrophic factor in the injured spinal cord, improved the pathological morphology at the injury site, reduced the loss of motor neurons, and promoted motor functional recovery in rats after spinal cord injury. Our experimental findings suggest that the neuroprotective effect of rapamycin intervention is mediated through activation of the Wnt/β-catenin signaling pathway after spinal cord injury.
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影响因子：
4.3
作者：
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通讯作者：
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DOI：
10.1016/j.ejvs.2014.02.006
发表时间：
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