The tobacco MAP215/Dis1-family protein TMBP200 is required for the functional organization of microtubule arrays during male germline establishment.
The tobacco MAP215/Dis1-family protein TMBP200 is required for the functional organization of microtubule arrays during male germline establishment.
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DOI：
10.1093/jxb/erp367
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发表时间：
2010-02
影响因子：
6.9
通讯作者：
Twell D
中科院分区：
文献类型：
--
作者：
Oh SA;Pal MD;Park SK;Johnson JA;Twell D
开花植物花粉发育过程中的单倍体小孢子分裂是一种本质上的不对称分裂，它建立了有性生殖的雄性生殖系。拟南芥gem 1突变体缺乏雄性生殖系的结果，扰乱小孢子极性，分裂不对称性，胞质分裂和代表MOR 1/GEM 1，保守的MAP 215/Dis 1微管相关蛋白（MAP）家族的植物直系同源物的功能丧失突变体。这为MAP 215/Dis 1在配子体微管阵列组织中的作用提供了遗传证据，但仍不清楚微管阵列在gem 1突变体小孢子中的影响。本研究构建了一种新的雄性配子体微管报告基因烟草（Nicotiana tabacum）植株，该植株在小孢子特异性启动子的控制下表达绿色荧光蛋白-α-微管蛋白融合蛋白（GFP-TUA 6）。这些植物允许所有主要的雄配子体微管阵列的有效可视化，并提供有用的工具来研究微管阵列的MAP和其他效应器的调节。耗尽TMBP 200，烟草同源的MOR 1/GEM 1在配子体微管报告植物，使用小孢子靶向RNA干扰，诱导小孢子极性，分裂不对称性和胞质分裂的缺陷，与成膜体的位置，方向和结构的显着缺陷。我们的观察进一步揭示了TMBP 200在配子体纺锤体组织中的需求，以及在极化小孢子中纺锤体位置和方向中的新作用。这些结果为MAP 215/Dis 1家族蛋白TMBP 200在组织微管阵列中的功能提供了直接证据，微管阵列对植物雄性生殖系的形成至关重要。
The haploid microspore division during pollen development in flowering plants is an intrinsically asymmetric division which establishes the male germline for sexual reproduction. Arabidopsis gem1 mutants lack the male germline as a result of disturbed microspore polarity, division asymmetry, and cytokinesis and represent loss-of-function mutants in MOR1/GEM1, a plant orthologue of the conserved MAP215/Dis1 microtubule associated protein (MAP) family. This provides genetic evidence for the role of MAP215/Dis1 in the organization of gametophytic microtubule arrays, but it has remained unknown how microtubule arrays are affected in gem1 mutant microspores. Here, novel male gametophytic microtubule-reporter Nicotiana tabacum plants were constructed, expressing a green fluorescent protein-α-TUBULIN fusion protein (GFP-TUA6) under the control of a microspore-specific promoter. These plants allow effective visualization of all major male gametophytic microtubule arrays and provide useful tools to study the regulation of microtubule arrays by MAPs and other effectors. Depletion of TMBP200, a tobacco homologue of MOR1/GEM1 in gametophytic microtubule-reporter plants using microspore-targeted RNA interference, induced defects in microspore polarity, division asymmetry and cytokinesis that were associated with striking defects in phragmoplast position, orientation, and structure. Our observations further reveal a requirement for TMBP200 in gametophytic spindle organization and a novel role in spindle position and orientation in polarized microspores. These results provide direct evidence for the function of MAP215/Dis1 family protein TMBP200 in the organization of microtubule arrays critical for male germline formation in plants.
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影响因子：
4.9
作者：
Eleftheriou, EP;Baskin, TI;Hepler, PK
通讯作者：
Hepler, PK
DOI：
10.1007/bf02488324
发表时间：
1987-06-01
期刊：
BOTANICAL MAGAZINE-TOKYO
影响因子：
--
作者：
TERASAKA, O;NIITSU, T
通讯作者：
NIITSU, T
影响因子：
7.4
作者：
Park, SK;Twell, D
通讯作者：
Twell, D
影响因子：
7.8
作者：
Shirasu-Hiza, Mimi;Coughlin, Peg;Mitchison, Tim
通讯作者：
Mitchison, Tim
影响因子：
64.5
作者：
Brouhard, Gary J.;Stear, Jeffrey H.;Hyman, Anthony A.
通讯作者：
Hyman, Anthony A.