External Qi of Yan Xin Qigong induces G2/M arrest and apoptosis of androgen-independent prostate cancer cells by inhibiting Akt and NF-κB pathways
External Qi of Yan Xin Qigong induces G2/M arrest and apoptosis of androgen-independent prostate cancer cells by inhibiting Akt and NF-κB pathways
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炎心气功外气通过抑制Akt和NF-κB通路诱导雄激素非依赖性前列腺癌细胞G2/M期阻滞和凋亡
DOI：
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发表时间：
2008
影响因子：
4.3
通讯作者：
Xinqi Wu
中科院分区：
文献类型：
--
作者：
Xin Yan;Hua Shen;Hongjian Jiang;Chengsheng Zhang;Dan Hu;Jun Wang;Xinqi Wu
长期的临床观察和正在进行的研究表明，延心气功(YXQG-EQ)的抗肿瘤作用源于中医。为了了解养血清前列腺癌EQ抗肿瘤作用的分子机制，我们研究了该药对雄激素非依赖性前列腺癌PC3细胞生长和凋亡的影响。我们发现，YXQG-EQ导致PC3细胞G2/M期停滞，并与细胞周期蛋白B1表达减少和细胞凋亡有关。养血清颗粒EQ可抑制原始性和表皮生长因子诱导的Akt磷酸化，抑制基础和肿瘤坏死因子-α诱导的NF-κB活化，并下调抗凋亡基因Bcl2和Bclxl的表达。相反，YXQG-EQ可增加人脐静脉内皮细胞(HUVEC)Akt和ERK1/2的磷酸化，而对HUVEC和外周血单核细胞(PBMC)均无细胞毒作用。这些结果表明，养血清颗粒EQ通过靶向AKT和NF-κB等生存通路，对前列腺癌细胞的生长和凋亡有深远的影响。
Long-term clinical observations and ongoing studies have shown antitumor effects of external Qi of Yan Xin Qigong (YXQG-EQ) that originated from traditional Chinese medicine (TCM). In order to understand the molecular mechanisms underlying the antitumor effects of YXQG-EQ, we investigate the effects of YXQG-EQ on growth and apoptosis in androgen-independent prostate cancer PC3 cells. We found that exposure to YXQG-EQ led to G2/M arrest associated with reduced cyclin B1 expression and apoptosis in PC3 cells. YXQG-EQ treatment inhibited constitutive and epidermal growth factor-induced Akt phosphorylation, basal and TNF-α-induced NF-κB activation, and downregulated anti-apoptotic Bcl-2 and Bcl-xL expression. In contrast, exposure to YXQG-EQ increased phosphorylation of Akt and Erk1/2 in human umbilical vein endothelial cells (HUVEC), and had no cytotoxic effect on either HUVEC or peripheral blood mononuclear cells (PBMC). These results indicate that YXQG-EQ has profound effects on growth and apoptosis of prostate cancer cells by targeting survival pathways including the Akt and NF-κB pathways.
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影响因子：
11.2
作者：
Yuxiang Zhang;Sanjeev Banerjee;Zhiwei Wang;Dorota J. Marciniak;A. Majumdar;F. Sarkar
通讯作者：
Yuxiang Zhang;Sanjeev Banerjee;Zhiwei Wang;Dorota J. Marciniak;A. Majumdar;F. Sarkar
XY99-5038 对培养的视网膜神经元中过氧化氢诱导的细胞死亡的保护作用。
影响因子：
6.1
作者：
Xin,Y;Fong,YT;Wolf,G;Wolf,D;Cao,W
通讯作者：
Cao,W
DOI：
10.1073/pnas.95.8.4386
发表时间：
1998-04-14
影响因子：
11.1
作者：
Hu, YM;Benedict, MA;Núñez, G
通讯作者：
Núñez, G
影响因子：
56.9
作者：
Cardone, MH;Roy, N;Reed, JC
通讯作者：
Reed, JC