Pilot ex vivo study on non-thermal ablation of human prostate adenocarcinoma tissue using boiling histotripsy.
Pilot ex vivo study on non-thermal ablation of human prostate adenocarcinoma tissue using boiling histotripsy.
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DOI：
10.1016/j.ultras.2023.107029
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发表时间：
2023-08
期刊：
影响因子：
4.2
通讯作者：
Khokhlova VA
中科院分区：
文献类型：
--
作者：
Rosnitskiy PB;Tsysar SA;Karzova MM;Buravkov SV;Malkov PG;Danilova NV;Ponomarchuk EM;Sapozhnikov OA;Khokhlova TD;Schade GR;Maxwell AD;Wang YN;Kadrev AV;Chernyaev AL;Okhobotov DA;Kamalov AA;Khokhlova VA
专注的超声技术对局部前列腺癌（PCA）的无创消融越来越感兴趣。在这里，我们介绍了第一个案例研究的结果，该案例研究评估了使用沸腾的组织疗法（BH）方法在体内组织中使用沸腾的腺癌组织非热机械消融的可行性。使用标称F＃= 0.75的1.5 MHz定制传感器生成高强度的超声场。在含有PCA的pCA的外体前列腺组织样品中测试了一种734 W声能，10毫秒长的BH-Puls，每个焦点的30脉冲，每个焦距30脉冲，每个焦距1％的脉冲，1％的占空比和1 mm的距离。此处使用的方案已成功地用于先前的BH研究中，用于用良性增生的体内前列腺人体组织的机械分解。使用B模式超声监测BH处理。治疗后的组织学分析表明，BH产生了靶向组织体积的液化。 BH处理过的良性前列腺实质和PCA具有类似的组织分级为亚细胞片段。该研究的结果表明，PCA肿瘤组织可以使用BH方法机械地消融。进一步的研究将旨在优化方案参数以加速处理，同时将目标组织体积彻底破坏到亚细胞碎片中。
Focused ultrasound technologies are of growing interest for noninvasive ablation of localized prostate cancer (PCa). Here we present the results of the first case study evaluating the feasibility of non-thermal mechanical ablation of human prostate adenocarcinoma tissue using the boiling histotripsy (BH) method on ex vivo tissue. High intensity focused ultrasound field was generated using a 1.5-MHz custom-made transducer with nominal F#=0.75. A sonication protocol of 734 W acoustic power, 10-ms long BH-pulses, 30 pulses per focal spot, 1% duty cycle, and 1 mm distance between single foci was tested in an ex vivo human prostate tissue sample containing PCa. The protocol used here has been successfully applied in the previous BH studies for mechanical disintegration of ex vivo prostatic human tissue with benign hyperplasia. BH treatment was monitored using B-mode ultrasound. Post-treatment histologic analysis demonstrated BH produced liquefaction of the targeted tissue volume. BH treated benign prostate parenchyma and PCa had similar tissue fractionation into subcellular fragments. The results of the study demonstrated that PCa tumor tissue can be mechanically ablated using the BH method. Further studies will aim on optimizing protocol parameters to accelerate treatment while maintaining complete destruction of the targeted tissue volume into subcellular debris.
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影响因子：
2.4
作者：
Khokhlova, Tatiana D.;Canney, Michael S.;Bailey, Michael R.
通讯作者：
Bailey, Michael R.
影响因子：
6.2
作者：
Litwin, Mark S.;Gore, John L.;Reiter, Robert E.
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Reiter, Robert E.
影响因子：
2.9
作者：
Nanda Kumar Y;Singh Z;Wang YN;Schade GR;Kreider W;Bruce M;Vlaisavljevich E;Khokhlova TD;Maxwell AD
通讯作者：
Maxwell AD
影响因子：
3.1
作者：
Eranki, Avinash;Farr, Navid;Wood, Bradford J.
通讯作者：
Wood, Bradford J.
影响因子：
2.9
作者：
Canney, Michael S.;Khokhlova, Vera A.;Bessonova, Olga V.;Bailey, Michael R.;Crum, Lawrence A.
通讯作者：
Crum, Lawrence A.