In vitro differentiation of leukemic cells to eosinophils in the presence of interleukin-5 in two cases of acute myeloid leukemia with the translocation (8;21)(q22;q22).
In vitro differentiation of leukemic cells to eosinophils in the presence of interleukin-5 in two cases of acute myeloid leukemia with the translocation (8;21)(q22;q22).
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在两例易位的急性髓系白血病中，在白细胞介素 5 存在的情况下，白血病细胞体外分化为嗜酸性粒细胞 (8;21)(q22;q22)。
DOI：
10.1182/blood.v75.2.350.350
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发表时间：
1990
期刊：
影响因子：
20.3
通讯作者：
Y. Miura
中科院分区：
文献类型：
--
作者：
H. Ema;K. Kitano;T. Suda;Y. Sato;K. Muroi;M. Ohta;M. Yoshida;S. Sakamoto;M. Eguchi;Y. Miura
我们证明了15例急性髓性白血病中有2例在白细胞介素-5（IL-5）存在下白血病细胞显著嗜酸性生长。这2例均为M2（FAB分类），易位为（8;21）（q22; q22）。骨髓检查显示，这两个病例的非典型嗜酸性粒细胞占总有核骨髓细胞的比例较高（6%和9%）。在其余13例中，嗜酸性粒细胞在有核骨髓细胞中小于2%。在甲基纤维素培养系统中，在这两种情况下，在存在IL-5的情况下，5 × 10（4）个单核细胞形成了142 +/- 18或54 +/- 2个集落。这些集落主要由成熟的嗜酸性粒细胞组成。通过Biebrich猩红染色和使用特异性凝集素结合试验的电子显微镜检查确认嗜酸性粒细胞。在液体培养系统中还观察到白血病细胞的嗜酸性分化和增殖。结果表明，在两种系统中观察到的嗜酸性粒细胞均来源于白血病细胞，使用白血病细胞的染色体标记t（8;21）。白血病细胞也分化为中性粒细胞或中性粒细胞和嗜酸性粒细胞分别响应于粒细胞集落刺激因子或白细胞介素-3，但没有显着响应于粒细胞-巨噬细胞集落刺激因子。虽然IL-5作为一种谱系特异性生长因子作用于正常的嗜酸性粒细胞定向前体细胞，但至少有部分白血病细胞对IL-5有反应，并可沿沿着嗜酸性粒细胞途径增殖和分化。我们的研究结果表明，在骨髓中观察到的非典型嗜酸性粒细胞来自两例AML的白血病克隆。
We demonstrated the significant eosinophilic growth of leukemic cells in the presence of interleukin-5 (IL-5) in 2 of 15 cases of acute myeloid leukemia. These two cases were M2 (FAB classification) with the translocation (8;21)(q22; q22). Bone marrow examination revealed the rather high percentages (6% and 9%) of atypical eosinophils in the total nucleated bone marrow cells in these two cases. In the remaining 13 cases, eosinophils were less than 2% in the nucleated bone marrow cells. In the methylcellulose culture system, 142 +/- 18 or 54 +/- 2 colonies were formed by 5 x 10(4) mononuclear cells in the presence of IL-5 in these two cases. These colonies mainly comprised mature eosinophils. Eosinophils were confirmed by Biebrich scarlet staining and electron microscopic examination using a specific lectin binding assay. The eosinophilic differentiation and proliferation of leukemic cells were also observed in the liquid culture system. It was shown that eosinophils observed in both systems were derived from leukemic cells using the chromosomal marker of leukemic cells, t(8;21). Leukemic cells also differentiated to neutrophils or both neutrophils and eosinophils in response to granulocyte colony-stimulating factor or interleukin-3, respectively, but did not respond noticeably to granulocyte-macrophage colony-stimulating factor. Although IL-5 acts on normal eosinophil committed precursors as a lineage-specific growth factor, at least some leukemic cells reacted to IL-5 and could proliferate and differentiate along eosinophilic pathway. Our findings suggest that atypical eosinophils observed in the bone marrow were derived from the leukemic clone in two cases of AML.
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影响因子：
20.3
作者：
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通讯作者：
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