Nitrite Triggers Reprogramming of the Oral Polymicrobial Metabolome by a Commensal Streptococcus.
Nitrite Triggers Reprogramming of the Oral Polymicrobial Metabolome by a Commensal Streptococcus.
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DOI：
10.3389/fcimb.2022.833339
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发表时间：
2022
影响因子：
5.7
通讯作者：
Scoffield JA
中科院分区：
文献类型：
--
作者：
Huffines JT;Stoner SN;Baty JJ;Scoffield JA
共生链球菌调节口腔内多种微生物群落的健康和稳态。值得注意的是，高唾液亚硝酸盐浓度也与口腔健康的改善有关。我们以前证明，亚硝酸盐协助过氧化氢生产口腔链球菌在调节体内平衡，通过产生活性氮物种（RNS），这对口腔病原体具有抗菌活性。然而，目前还不清楚亚硝酸盐和链球菌如何协同作用，影响口腔多微生物群落的代谢组。在这项研究中，我们报告说，亚硝酸盐艾滋病链球菌在抑制多王国的病原体，居住在不同的口腔壁龛，这支持adhesal优势。更重要的是，我们表明，亚硝酸盐链球菌利用亚硝酸盐来驱动多物种生物膜的代谢特征，支持亚硝酸盐代谢和抵抗RNS的方式，并限制病原体毒力所需的代谢过程。综上所述，我们的研究提供了对口腔链球菌如何利用亚硝酸盐来触发口腔多微生物代谢组的变化以支持健康和体内平衡的见解。
Commensal streptococci regulate health and homeostasis within oral polymicrobial communities. Remarkably, high salivary nitrite concentrations have also been associated with improved health in the oral cavity. We previously demonstrated that nitrite assists hydrogen peroxide-producing oral commensal streptococci in regulating homeostasis via the generation of reactive nitrogen species (RNS), which have antimicrobial activity on oral pathogens. However, it is unknown how nitrite and commensal streptococci work in concert to influence the metabolome of oral polymicrobial communities. In this study, we report that nitrite aids commensal streptococci in the inhibition of multi-kingdom pathogens that reside in distinct oral niches, which supports commensal dominance. More importantly, we show that commensal streptococci utilize nitrite to drive the metabolic signature of multispecies biofilms in a manner that supports commensal metabolism and resistance to RNS, and restricts metabolic processes that are required for pathogen virulence. Taken together, our study provides insight into how commensal streptococci use nitrite to trigger shifts in the oral polymicrobial metabolome to support health and homeostasis.
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