Comprehensive analysis of clinical significance of stem-cell related factors in renal cell cancer.
Comprehensive analysis of clinical significance of stem-cell related factors in renal cell cancer.
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DOI：
10.1186/1477-7819-9-121
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发表时间：
2011-10-07
影响因子：
3.2
通讯作者：
Xia S
中科院分区：
文献类型：
--
作者：
Liu Y;Zhang C;Fan J;Xiao L;Yin B;Zhou L;Xia S
C-MYC、LIN28、OCT4、KLF4、NANOG、SOX2是干细胞相关因子。我们检测这些因子是否在肾细胞癌（RCC）组织中表达，以研究它们与临床和病理特征的相关性。应用实时定量逆转录pcr （qRT-PCR）检测30例RCC患者和5例非RCC患者中c-MYC、LIN28、SOX2、KLF4、OCT4和NANOG的表达。采用Wilcoxon符号秩和检验和x2检验对数据进行分析。RCC组c-MYC在RCC组织中的表达明显高于正常组织（P < 0.05）。OCT4、KLF4、NANOG、SOX2在RCC组织中的表达水平明显低于正常组织（P < 0.05）。LIN28表达量无统计学意义。两组患者在性别、年龄、肿瘤大小、cTNM分型及分化情况等临床病理特征上差异无统计学意义（P < 0.05）。非rcc组上述因子的表达水平无显著变化（P < 0.05）。目前的分析强烈表明，几种干细胞相关因子的表达改变可能在RCC中发挥不同的作用。C-MYC可能作为癌基因，OCT4、KLF4、NANOG和SOX2可能作为抑癌基因。
C-MYC, LIN28, OCT4, KLF4, NANOG and SOX2 are stem cell related factors. We detected whether these factors express in renal cell carcinoma (RCC) tissues to study their correlations with the clinical and pathological characteristics. The expressions of c-MYC, LIN28, SOX2, KLF4, OCT4 and NANOG in 30 RCC patients and 5 non-RCC patients were detected with quantitative real-time reverse transcription-PCR (qRT-PCR). The data were analyzed with Wilcoxon signed rank sum test and x2 test. In RCC group, c-MYC expression was significantly higher in RCC tissues compared with normal tissues (P < 0.05). The expression levels of OCT4, KLF4, NANOG and SOX2 were significantly lower in RCC tissues compared with normal tissues (P < 0.05). LIN28 expression level was not significant. No difference was observed when it comes to clinical and pathological characteristics such as gender, age, tumor size, cTNM classification and differentiation status (P > 0.05). Also the expression levels of all above factors were not significantly changed in non-RCC group (P > 0.05). The present analysis strongly suggests that altered expression of several stem cell related factors may play different roles in RCC. C-MYC may function as an oncogene and OCT4, KLF4, NANOG and SOX2 as tumor suppressors.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
4.8
作者：
Bussolati, Benedetta;Bruno, Stefania;Camussi, Giovanni
通讯作者：
Camussi, Giovanni
影响因子：
11.2
作者：
Sampson, Valerie B.;Rong, Nancy H.;Krueger, Leslie J.
通讯作者：
Krueger, Leslie J.
影响因子：
8
作者：
Foster, KW;Liu, ZL;Ruppert, JM
通讯作者：
Ruppert, JM
影响因子：
7.5
作者：
Moreira, Andre L.;Gonen, Mithat;Downey, Robert J.
通讯作者：
Downey, Robert J.