Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS.
Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS.
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DOI：
10.1002/anie.201802843
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发表时间：
2018-09-17
期刊：
影响因子：
--
通讯作者：
Kelly RT
中科院分区：
文献类型：
--
作者：
Zhu Y;Clair G;Chrisler WB;Shen Y;Zhao R;Shukla AK;Moore RJ;Misra RS;Pryhuber GS;Smith RD;Ansong C;Kelly RT
We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was employed to deposit cells into a newly developed nano-droplet sample processing chip, after which samples were analyzed by ultrasensitive nanoLC-MS. An average of circa 670 protein groups were confidently identified from single HeLa cells, which is a far greater level of proteome coverage for single cells than has been previously reported. We demonstrate that the single-cell proteomics platform can be used to differentiate cell types from enzyme-dissociated human lung primary cells and identify specific protein markers for epithelial and mesenchymal cells.
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影响因子：
64.8
作者：
Halpern KB;Shenhav R;Matcovitch-Natan O;Toth B;Lemze D;Golan M;Massasa EE;Baydatch S;Landen S;Moor AE;Brandis A;Giladi A;Avihail AS;David E;Amit I;Itzkovitz S
通讯作者：
Itzkovitz S
DOI：
10.1084/jem.20170976
发表时间：
2017-10-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Tang Q;Iyer S;Lobbardi R;Moore JC;Chen H;Lareau C;Hebert C;Shaw ML;Neftel C;Suva ML;Ceol CJ;Bernards A;Aryee M;Pinello L;Drummond IA;Langenau DM
通讯作者：
Langenau DM
影响因子：
7
作者：
Li, Siyang;Plouffe, Brian D.;Ivanov, Alexander R.
通讯作者：
Ivanov, Alexander R.
通过直接细胞注射、在线消化和 Nano-LC-MS/MS 分析对 100 个活细胞进行超灵敏蛋白质组分析
影响因子：
7.4
作者：
Chen, Qi;Yan, Guoquan;Zhang, Xiangmin
通讯作者：
Zhang, Xiangmin
影响因子：
5.8
作者：
Le, Sebastien;Josse, Julie;Husson, Francois
通讯作者：
Husson, Francois