Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
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DOI：
10.1084/jem.20170976
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发表时间：
2017-10-02
期刊：
影响因子：
--
通讯作者：
Langenau DM
中科院分区：
文献类型：
--
作者：
Tang Q;Iyer S;Lobbardi R;Moore JC;Chen H;Lareau C;Hebert C;Shaw ML;Neftel C;Suva ML;Ceol CJ;Bernards A;Aryee M;Pinello L;Drummond IA;Langenau DM
Tang等人的工作对成年斑马鱼的肾脏和血细胞进行了全面的单细胞转录组分析，鉴定了新型细胞类型，包括两类NK免疫细胞、经典定义的和红细胞致敏的造血干细胞和祖细胞、粘液分泌肾细胞和肾干细胞/祖细胞。单细胞转录组学分析的最新进展为研究组织和器官发育过程中的细胞异质性提供了前所未有的途径。在这项研究中，我们使用大规模平行，单细胞RNA测序来定义斑马鱼肾骨髓内的细胞异质性，构建了一个全面的成熟的造血和功能不同的肾细胞在成年斑马鱼中发现的分子图谱。由于我们的方法以无偏的方式分析了血液和肾细胞，因此我们的方法可用于表征DNA-蛋白激酶催化亚基（prkdc）、白细胞介素-2受体γ a（il 2 rga）和双纯合突变鱼中的免疫细胞缺陷，鉴定特定遗传突变体中T、B和自然杀伤细胞中的血细胞损失。我们的分析还发现了新的细胞类型，包括两类自然杀伤免疫细胞，经典定义和红系引发的造血干细胞和祖细胞，分泌粘蛋白的肾细胞和肾干/祖细胞。总的来说，我们的工作提供了第一个，全面的，单细胞，转录组分析的肾脏和骨髓细胞在成年斑马鱼。
The work by Tang et al. provides a comprehensive, single-cell, transcriptomic analysis of kidney and blood cells from the adult zebrafish, identifying novel cell types, including two classes of NK immune cells, classically defined and erythroid-primed hematopoietic stem and progenitor cells, mucin-secreting kidney cells, and kidney stem/progenitor cells. Recent advances in single-cell, transcriptomic profiling have provided unprecedented access to investigate cell heterogeneity during tissue and organ development. In this study, we used massively parallel, single-cell RNA sequencing to define cell heterogeneity within the zebrafish kidney marrow, constructing a comprehensive molecular atlas of definitive hematopoiesis and functionally distinct renal cells found in adult zebrafish. Because our method analyzed blood and kidney cells in an unbiased manner, our approach was useful in characterizing immune-cell deficiencies within DNA–protein kinase catalytic subunit (prkdc), interleukin-2 receptor γ a (il2rga), and double-homozygous–mutant fish, identifying blood cell losses in T, B, and natural killer cells within specific genetic mutants. Our analysis also uncovered novel cell types, including two classes of natural killer immune cells, classically defined and erythroid-primed hematopoietic stem and progenitor cells, mucin-secreting kidney cells, and kidney stem/progenitor cells. In total, our work provides the first, comprehensive, single-cell, transcriptomic analysis of kidney and marrow cells in the adult zebrafish.
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