Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology.
Orthotopic mouse lung transplantation as experimental methodology to study transplant and tumor biology.
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DOI：
10.1038/nprot.2008.218
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发表时间：
2009
期刊：
影响因子：
14.8
通讯作者：
Kreisel, Daniel
中科院分区：
文献类型：
--
作者：
Krupnick, Alexander S.;Lin, Xue;Li, Wenjun;Okazaki, Mikio;Lai, Jiaming;Sugimoto, Seiichiro;Richardson, Steven B.;Kornfeld, Christopher G.;Garbow, Joel R.;Patterson, G. Alexander;Gelman, Andrew E.;Kreisel, Daniel
与其他实体器官不同，带血管的小鼠肺移植是最近才发展起来的。在这篇文章中，我们描述了进行血管化和充气小鼠原位肺移植的详细方法，这是迄今为止最具生理性的小鼠肺移植模型。该手术分为两个独立的部分，包括供体收获，然后使用袖带技术进行支气管血管吻合植入。经过几个月的训练，程序可以成功地掌握和在有经验的手大约需要90分钟来执行。经过最初的学习曲线后，围手术期生存率接近100%。随着时间的推移，移植肺中的供体造血细胞被宿主的取代，从而产生“嵌合肺”，该模型代表了移植生物学研究以及其他疾病过程（如恶性肿瘤）的新研究工具。
Unlike other solid organs, vascularized mouse lung transplantation has only recently been developed. In this publication we describe the detailed method for performing a vascularized and aerated mouse orthotopic lung transplant, which to date represents the most physiologic mouse model of lung transplantation. The procedure is divided into two separate portions consisting of donor harvest followed by implantation using the cuff technique for bronchovascular anastomoses. After a several month training period the procedure can be successfully mastered and in experienced hands requires approximately 90 minutes to perform. After an initial learning curve perioperative survival is close to 100%. As over time the donor hematopoietic cells in the transplanted lung are replaced by those of the host, thereby creating a “chimeric lung”, this model represents a novel research tool for the study of transplantation biology as well as other disease processes such as malignancies.
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影响因子：
4.4
作者：
Gelman, Andrew E.;Okazaki, Mikio;Kreisel, Daniel
通讯作者：
Kreisel, Daniel
DOI：
10.1165/rcmb.2007-0257rc
发表时间：
2007-12-01
影响因子：
6.4
作者：
Okazaki, Mikio;Gelman, Andrew E.;Kreisel, Daniel
通讯作者：
Kreisel, Daniel
影响因子：
158.5
作者：
Iacono, AT;Johnson, BA;Griffith, BP
通讯作者：
Griffith, BP
影响因子：
8.8
作者：
Truong, W.;Emamaullee, J. A.;Shapiro, A. M. James
通讯作者：
Shapiro, A. M. James
DOI：
10.1084/jem.184.5.2013
发表时间：
1996-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Krieger NR;Yin DP;Fathman CG
通讯作者：
Fathman CG