Developmental maturation of excitation and inhibition balance in principal neurons across four layers of somatosensory cortex.
Developmental maturation of excitation and inhibition balance in principal neurons across four layers of somatosensory cortex.
复制标题
体感皮层四层主要神经元兴奋和抑制平衡的发育成熟。
DOI：
10.1016/j.neuroscience.2010.11.045
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发表时间：
2011-02-03
期刊：
影响因子：
3.3
通讯作者：
Sun, Q. -Q.
中科院分区：
文献类型：
--
作者：
Zhang, Z.;Jiao, Y. -Y.;Sun, Q. -Q.
在成人皮层中，兴奋性和抑制性电导的比率（E/I比率）可能在广泛的刺激条件下保持平衡。然而，当突触的强度迅速变化时，E/I比率是如何在出生后调节的尚不清楚。然而，了解这一过程至关重要，因为有许多神经心理疾病，如自闭症、癫痫和精神分裂症，都与E/I平衡紊乱有关。在这里，我们直接测量了从出生后第8天到第60天主要神经元局部诱导突触传导的E/I比率。结果表明：1)在不同的发育时期，在较宽的刺激强度范围内，4个主要皮质层的E/I比值保持在相近的水平；2)除第II/III层外，E/I比在第8 ~ 18层呈快速发育性下降。通过比较兴奋性和抑制性电导以及关键突触蛋白的表达，我们发现抑制突触的数量和强度的净增加，而不是兴奋性突触的净增加，是导致桶状皮质E/I比率发育性下降的原因。抑制标记本质上是共同调节的，导致从P8到P18的抑制电导急剧增加。这些结果表明，成人皮层严格调节的E/I比率是在关键时期抑制性突触而非兴奋性突触相对重量剧烈变化的结果，局部抑制性结构变化是出生后发育过程中E/I比率改变的基础。
In adult cortices, the ratio of excitatory and inhibitory conductances (E/I ratio) is presumably balanced across a wide range of stimulus conditions. However, it is unknown how the E/I ratio is postnatally regulated, when the strength of synapses are rapidly changing. Yet, understanding of such a process is critically important, because there are numerous neuropsychological disorders, such as autism, epilepsy and schizophrenia, are associated with disturbed E/I balances. Here we directly measured the E/I ratio underlying locally induced synaptic conductances in principal neurons from postnatal day 8 through 60. We found that 1) within each developmental period, the E/I ratio across 4 major cortical layers was maintained at a similar value under wide range of stimulation intensities; and 2) there was a rapid developmental decrease in the E/I ratio, which occurred within a sensitive period between P8 to P18 with exception of layer II/III. By comparing the excitatory and inhibitory conductances, as well as key synaptic protein expressions, we found a net increase in the number and strength of inhibitory, but not excitatory synapses, is responsible for the developmental decrease in the E/I ratio in the barrel cortex. The inhibitory markers were intrinsically co-regulated, gave rise to a sharp increase in the inhibitory conductance from P8 to P18. These results suggest that the tightly regulated E/I ratios in adults cortex is a result of drastic changes in relative weight of inhibitory but not excitatory synapses during critical period, and the local inhibitory structural changes are the underpinning of altered E/I ratio across postnatal development.
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影响因子：
25
作者：
Cruikshank, Scott J.;Lewis, Timothy J.;Connors, Barry W.
通讯作者：
Connors, Barry W.
兴奋性和抑制性可塑性对突触驱动的神经元输出输出功能的差异影响。
影响因子：
16.2
作者：
Carvalho, Tiago P.;Buonomano, Dean V.
通讯作者：
Buonomano, Dean V.
影响因子：
64.8
作者：
CRAIR, MC;MALENKA, RC
通讯作者：
MALENKA, RC
影响因子：
5.3
作者：
Brill, Julia;Huguenard, John R.
通讯作者：
Huguenard, John R.
影响因子：
56.9
作者：
BOLSHAKOV, VY;SIEGELBAUM, SA
通讯作者：
SIEGELBAUM, SA