Ara h 2 and Ara h 6 have similar allergenic activity and are substantially redundant.
Ara h 2 and Ara h 6 have similar allergenic activity and are substantially redundant.
复制标题
DOI：
10.1159/000341642
复制
发表时间：
2013
影响因子：
2.8
通讯作者：
Dreskin SC
中科院分区：
文献类型：
--
作者：
Chen X;Wang Q;El-Mezayen R;Zhuang Y;Dreskin SC
中等同源性 (~60%) 的蛋白质 Ara h 2 和 Ara h 6 是最有效的花生过敏原。本研究旨在确定 Ara h 2 和 Ara h 6 对花生粗提取物 (CPE) 总体过敏活性的相对个体贡献。通过凝胶过滤色谱从CPE中除去Ara h 2和Ara h 6。 Ara h 2.01、Ara h 2.02 和 Ara h 6 进一步纯化 (>99%)。用来自致敏花生过敏患者的 IgE 致敏的 RBL SX-38 细胞测量每种过敏原的效力以及这些过敏原重建耗尽 Ara h 2 和 Ara h 6 的 CPE 的过敏活性的能力。天然蛋白的效力有显着差异 (p<0.0001)，但差异并不显着，排名顺序为 Ara h 2.01 > Ara h 2.02 > Ara h 6。将纯化的 Ara h 2 或 Ara h 6 以其原始浓度独立添加到耗尽 Ara h 2 和 Ara h 6 的 CPE 中，可恢复 80-100% 的原始 CPE 过敏活性。添加 Ara h 2 和 Ara h 6 始终能够完全恢复 CPE 的过敏活性。这些研究表明，Ara h 2 或 Ara h 6 可以独立地解释 CPE 中的大部分过敏活性，并证明这些相关分子的过敏活性具有重要的冗余性。
The moderately homologous (~60%) proteins, Ara h 2 and Ara h 6, are the most potent peanut allergens. This study was designed to define the relative individual contributions of Ara h 2 and Ara h 6 to the overall allergenic activity of a crude peanut extract (CPE). Ara h 2 and Ara h 6 were removed from CPE by gel filtration chromatography. Ara h 2.01, Ara h 2.02, and Ara h 6 were further purified (>99%). The potency of each allergen and the ability of these allergens to reconstitute the allergenic activity of CPE depleted of Ara h 2 and Ara h 6 was measured with RBL SX-38 cells sensitized with IgE from sensitized peanut allergic patients. The potency of the native proteins were significantly different (p<0.0001) although not dramatically so, with a rank order of Ara h 2.01 > Ara h 2.02 > Ara h 6. The addition of either purified Ara h 2 or Ara h 6 independently at their original concentration to CPE depleted of both Ara h 2 and Ara h 6 restored 80–100% of the original CPE allergenic activity. Addition of both Ara h 2 and Ara h 6 consistently completely restored the allergenic activity of CPE. These studies indicate that either Ara h 2 or Ara h 6 independently can account for most of the allergenic activity in a CPE and demonstrate important redundancy in the allergenic activity of these related molecules.
登录
查看更多内容
影响因子：
14.2
作者：
Shreffler, WG;Lencer, DA;Sampson, HA
通讯作者：
Sampson, HA
影响因子：
14.2
作者：
BURKS, AW;WILLIAMS, LW;HELM, RM
通讯作者：
HELM, RM
影响因子：
6.1
作者：
McDermott, R. A.;Porterfield, H. S.;Dreskin, S. C.
通讯作者：
Dreskin, S. C.
影响因子：
4.4
作者：
Sen, M;Kopper, R;Bannon, GA
通讯作者：
Bannon, GA
影响因子：
2.8
作者：
Hales, BJ;Bosco, A;Thomas, WR
通讯作者：
Thomas, WR